Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Dec;112(3):1353–1357. doi: 10.1128/jb.112.3.1353-1357.1972

Phenoxazinone Synthetase from Streptomyces antibioticus: Multiple Activities of the Enzyme

Ellis E Golub a,1, Jonathan S Nishimura a
PMCID: PMC251570  PMID: 4118295

Abstract

A procedure for the preparation of relatively large quantities of highly purified phenoxazinone synthetase from Streptomyces antibioticus is described. Enzyme preparations consisted of multiple forms, as determined by polyacrylamide gel electrophoresis. Each of the electrophoretically separable forms catalyzed the oxidation of catechols, ferrocyanide, and ethylenic thiols, in addition to o-aminophenols.

Full text

PDF
1353

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AURBACH G. D., JAKOBY W. B. The multiple functions of thiooxidase. J Biol Chem. 1962 Feb;237:565–568. [PubMed] [Google Scholar]
  2. BOUCHILLOUX S., McMAHILL P., MASON H. S. The multiple forms of mushroom tyrosinase. Purification and molecular properties of the enzymes. J Biol Chem. 1963 May;238:1699–1707. [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. FLING M., HOROWITZ N. H., HEINEMANN S. F. The isolation and properties of crystalline tyrosinase from Neurospora. J Biol Chem. 1963 Jun;238:2045–2053. [PubMed] [Google Scholar]
  5. Friedman S., Kaufman S. 3,4-dihydroxyphenylethylamine beta-hydroxylase. Physical properties, copper content, and role of copper in the catalytic acttivity. J Biol Chem. 1965 Dec;240(12):4763–4773. [PubMed] [Google Scholar]
  6. Friedman S., Kaufman S. An electron paramagnetic resonance study of 3,4-dihydroxyphenylethylamine beta-hydroxylase. J Biol Chem. 1966 May 25;241(10):2256–2259. [PubMed] [Google Scholar]
  7. GOSS W. A., KATZ E. Actinomycin formation by Streptomyces cultures. Appl Microbiol. 1957 Mar;5(2):95–102. doi: 10.1128/am.5.2.95-102.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jolley R. L., Jr, Robb D. A., Mason H. S. The multiple forms of mushroom tyrosinase. Association-dissociation phenomena. J Biol Chem. 1969 Mar 25;244(6):1593–1599. [PubMed] [Google Scholar]
  9. KATZ E., WEISSBACH H. Biosynthesis of the actinomycin chromophore; enzymatic conversion of 4-methyl-3-hydroxyanthranilic acid to actinocin. J Biol Chem. 1962 Mar;237:882–886. [PubMed] [Google Scholar]
  10. LEVIN E. Y., KAUFMAN S. Studies on the enzyme catalyzing the conversion of 3,4-dihydroxyphenylethylamine to norepinephrine. J Biol Chem. 1961 Jul;236:2043–2049. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Nishimura J. S., Golub E. E. On the effects of cyanide on phenoxazinone synthetase. Biochim Biophys Acta. 1969;191(3):724–726. doi: 10.1016/0005-2744(69)90369-6. [DOI] [PubMed] [Google Scholar]
  13. SWINGLE S. M., TISELIUS A. Tricalcium phosphate as an adsorbent in the chromatography of proteins. Biochem J. 1951 Feb;48(2):171–174. doi: 10.1042/bj0480171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Salzman L., Weissbach H., Katz E. Enzymatic synthesis of actinocinyl peptides. Arch Biochem Biophys. 1969 Mar;130(1):536–546. doi: 10.1016/0003-9861(69)90067-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES