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. 1972 Dec;112(3):1375–1379. doi: 10.1128/jb.112.3.1375-1379.1972

Membrane Association of the Deoxyribonucleic Acid Growing-Point Region in Mycoplasma gallisepticum

Dennis C Quinlan 1, Jack Maniloff 2
PMCID: PMC251573  PMID: 4264449

Abstract

Newly replicated deoxyribonucleic acid (DNA) in Mycoplasma gallisepticum A5969 is membrane associated. Cells pulse-labeled 1 to 3 min with 3H-thymidine are lysed by a freeze-thaw procedure. After brief sonic treatment to shear the DNA, differential centrifugation gives a cell fraction (P2) that is enriched sevenfold for pulse-labeled DNA. P2 contains 80% of the total adenosine triphosphatase activity, 65% of the total cholesterol, and morphologically intact terminal bleb structures. Three to four minutes are needed to fully label the DNA growing-point region, whereas 7 to 8 min are required to “chase” 50% of the 3H-labeled DNA. This pulse-chase removes 80 to 85% of the nascent DNA from the P2 fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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