Abstract
Extracellular d-fructose was metabolized preferentially over intracellular d-fructose in growing cells of a d-fructokinaseless mutant of Aerobacter aerogenes. This finding is interpreted to indicate that phosphorylation of d-fructose by the membrane-bound phosphoenolpyruvate: d-fructose 1-phosphotransferase system in intact cells is coupled to unidirectional transport.
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Selected References
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- Hanson T. E., Anderson R. L. D-fructose 1-phosphate kinase, a new enzyme instrumental in the metabolism of D-fructose. J Biol Chem. 1966 Apr 10;241(7):1644–1645. [PubMed] [Google Scholar]
- Hanson T. E., Anderson R. L. Phosphoenolpyruvate-dependent formation of D-fructose 1-phosphate by a four-component phosphotransferase system. Proc Natl Acad Sci U S A. 1968 Sep;61(1):269–276. doi: 10.1073/pnas.61.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaback H. R. The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli. J Biol Chem. 1968 Jul 10;243(13):3711–3724. [PubMed] [Google Scholar]
- Kaback H. R. Transport. Annu Rev Biochem. 1970;39:561–598. doi: 10.1146/annurev.bi.39.070170.003021. [DOI] [PubMed] [Google Scholar]
- Kelker N. E., Hanson T. E., Anderson R. L. Alternate pathways of D-fructose metabolism in Aerobacter aerogenes. A specific D-fructokinase and its preferential role in the metabolism of sucrose. J Biol Chem. 1970 Apr 25;245(8):2060–2065. [PubMed] [Google Scholar]
- Sapico V., Anderson R. L. D-fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties. J Biol Chem. 1969 Nov 25;244(22):6280–6288. [PubMed] [Google Scholar]
- Sapico V., Anderson R. L. Regulation of D-fructose 1-phosphate kinase by potassium ion. J Biol Chem. 1970 Jun;245(12):3252–3256. [PubMed] [Google Scholar]
- Sapico V., Hanson T. E., Walter R. W., Anderson R. L. Metabolism of D-fructose in Aerobacter aerogenes: analysis of mutants lacking D-fructose 6-phosphate kinase and D-fructose 1,6-diphosphatase. J Bacteriol. 1968 Jul;96(1):51–54. doi: 10.1128/jb.96.1.51-54.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]