Figure 2.—

Quantitative reporter plating assay for spread of silencing. Serial platings of mutant strains show increased growth on 5-FOA media, assembled into subcategories. Each plate contains a ura3Δ strain as a positive control, and a reporter strain bearing no knockouts (wild type) as a negative control, and an htz1Δ mutation in the reporter strain as an example of an ectopic silencing phenotype. (A) SAS-I complex and Mediator components. (B) Rpd3-L complex components. (C) Telomere maintenance genes. (D) Genes affecting protein phosphorylation and others. (E) Swr1 and Rtt109 complex components. (F) Metabolism-linked genes.