Abstract
The phytohemagglutinin, concanavalin A (Con A), interacts specifically and reversibly with the α-d-glucose-substituted polyglycerol phosphate teichoic acids of Bacillus subtilis 168. Teichoic acids from nonglucosylated strains (mutants resistant to phage φ25) interact weakly, if at all, with Con A. Prior treatment of glucosylated cell walls with Con A results in a concentration-dependent inhibition of phage φ25 adsorption. The inhibition of adsorption to glucosylated cell walls occurs immediately upon the formation of the Con A-cell wall complex and is reversed upon dissociation of the complex by addition of α-methyl glucose. Examination of the interaction between Con A and whole cells, cell walls, or wall digests from glucosylated (phage-sensitive) and non-glucosylated (phage-resistant) strains further demonstrates the specificity of the reaction. A conditional phage φ25-resistant mutant only forms complexes with Con A when grown under permissive conditions.
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- Archibald A. R., Coapes H. E. The interaction of concanavalin A with teichoic acids and bacterial walls. Biochem J. 1971 Jul;123(4):665–667. doi: 10.1042/bj1230665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doyle R. J., Birdsell D. C. Interaction of concanavalin A with the cell wall of Bacillus subtilis. J Bacteriol. 1972 Feb;109(2):652–658. doi: 10.1128/jb.109.2.652-658.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mauck J., Chan L., Glaser L. Turnover of the cell wall of Gram-positive bacteria. J Biol Chem. 1971 Mar 25;246(6):1820–1827. [PubMed] [Google Scholar]
- ROE J. H. The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem. 1955 Jan;212(1):335–343. [PubMed] [Google Scholar]
- Reeder W. J., Ekstedt R. D. Study of the interaction of concanavalin A with staphylocccal teichoic acids. J Immunol. 1971 Feb;106(2):334–340. [PubMed] [Google Scholar]
- Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]
- Young F. E. Requirement of glucosylated teichoic acid for adsorption of phage in Bacillus subtilis 168. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2377–2384. doi: 10.1073/pnas.58.6.2377. [DOI] [PMC free article] [PubMed] [Google Scholar]