Fig. 3.
ST induces CaR translocation in colon cancer cells. (A) Intracellular cGMP accumulation induced by ST (1 μM for 15 min) in T84 cells pretreated with ST (1 μM for 24 h) or the vehicle control in the presence of the indicated Ca2+o concentration. *P < 0.05 versus respective control conditions not pretreated with ST. (B) Time course of ST (1 μM) effects on cytosol (upper panel) or membrane (lower panel) CaR expression in T84 cells. Phosphate-buffered saline (PBS), the vehicle control; GAPDH and villin, the loading controls.