Abstract
Yeast killer factor proteins bind to cells of both sensitive and killer-producing strains, although the latter are immune to killer action. Spheroplasts prepared from sensitive cells bind less than 1% of the killer bound to whole cells, but remain fully sensitive to killer. This finding and those obtained from binding studies of partially purified, radioactive killer protein suggest that most of the toxins remain bound to the yeast cell wall and do not function further in the killing process. A killer-resistant mutant R18 was isolated from a sensitive strain. Whole cells of the mutant were unable to bind killer and were fully resistant. In contrast, spheroplasts of R18 were fully sensitive to killer. These data suggest that the sites exposed to killer in spheroplasts are distinct from those on the cell wall. These wall sites appear to be necessary for killer action in whole cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bussey H. Effects of yeast killer factor on sensitive cells. Nat New Biol. 1972 Jan 19;235(55):73–75. doi: 10.1038/newbio235073a0. [DOI] [PubMed] [Google Scholar]
- HALVORSON H. Studies on protein and nucleic acid turnover in growing cultures of yeast. Biochim Biophys Acta. 1958 Feb;27(2):267–276. doi: 10.1016/0006-3002(58)90333-0. [DOI] [PubMed] [Google Scholar]
- Hutchison H. T., Hartwell L. H. Macromolecule synthesis in yeast spheroplasts. J Bacteriol. 1967 Nov;94(5):1697–1705. doi: 10.1128/jb.94.5.1697-1705.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nagel de Zwaig R. Mode of action of colicin A. J Bacteriol. 1969 Sep;99(3):913–914. doi: 10.1128/jb.99.3.913-914.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Somers J. M., Bevan E. A. The inheritance of the killer character in yeast. Genet Res. 1969 Feb;13(1):71–83. doi: 10.1017/s0016672300002743. [DOI] [PubMed] [Google Scholar]
- Woods D. R., Bevan E. A. Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J Gen Microbiol. 1968 Apr;51(1):115–126. doi: 10.1099/00221287-51-1-115. [DOI] [PubMed] [Google Scholar]