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. 1973 Mar;113(3):1264–1267. doi: 10.1128/jb.113.3.1264-1267.1973

Resolution of the Nitrate Reductase Complex from the Membrane of Escherichia coli

E I Villarreal-Moguel 1, V Ibarra 1, J Ruíz-Herrera 1, C Gitler 1
PMCID: PMC251692  PMID: 4570777

Abstract

A procedure has been developed whereby the components of the nitrate reductase complex from the membrane of Escherichia coli can be extracted by means of a nonionic detergent and separated from each other as discrete elements in an active form. It is not yet clear whether these are the dissociated enzymatic elements or whether they are fragments containing other membrane proteins. In any case, they appeared nearly lipid-free and still active.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  2. Morris D. W., DeMoss J. A. Polysome transitions and the regulation of ribonucleic acid synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1966 Jul;56(1):262–268. doi: 10.1073/pnas.56.1.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ruiz-Herrera J., DeMoss J. A. Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction. J Bacteriol. 1969 Sep;99(3):720–729. doi: 10.1128/jb.99.3.720-729.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ruiz-Herrera J., Showe M. K., DeMoss J. A. Nitrate reductase complex of Escherichia coli K-12: isolation and characterization of mutants unable to reduce nitrate. J Bacteriol. 1969 Mar;97(3):1291–1297. doi: 10.1128/jb.97.3.1291-1297.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Showe M. K., DeMoss J. A. Localization and regulation of synthesis of nitrate reductase in Escherichia coli. J Bacteriol. 1968 Apr;95(4):1305–1313. doi: 10.1128/jb.95.4.1305-1313.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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