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. 1973 Mar;113(3):1320–1325. doi: 10.1128/jb.113.3.1320-1325.1973

Histidine Uptake in Mutant Strains of Neurospora crassa Via the General Transport System for Amino Acids

C W Magill 1, S O Nelson 1, S M D'Ambrosio 1, G I Glover 1
PMCID: PMC251700  PMID: 4266243

Abstract

A transport double mutant of Neurospora crassa has been isolated that has only one of the three transport systems capable of l-histidine uptake. The substrate specificity of the remaining transport system, termed the general transport system, has been fully characterized with regard to the contributions to binding of the side chain, the α-amino group, and the carboxylate group. The positively charged α-amino group is necessary for binding; the negatively charged carboxylate group is of less importance, since its replacement by a neutral carbonyl functional group does not completely abolish binding. The greatest structural latitude for binding was found in the side chain; affinity for α-amino acids was uniformly high except for l-aspartic and l-glutamic acids, l-asparagine, and l-proline. Thus, this transport system is “general” with these restrictions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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