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. 1973 Mar;113(3):1363–1372. doi: 10.1128/jb.113.3.1363-1372.1973

Manganese Transport in Bacillus subtilis W23 During Growth and Sporulation

Eric Eisenstadt a,1, Susan Fisher a,2, Chi-Lui Der a, Simon Silver a
PMCID: PMC251706  PMID: 4632400

Abstract

Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element “pump” is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentachlorophenol, and m-chlorophenyl carbonylcyanide hydrazone. The uptake of manganese follows Michaelis-Menten kinetics, and the net accumulation of manganese is regulated by increasing the Vmax after exposure to manganese-starvation conditions and by decreasing the Vmax for manganese uptake during growth in excess manganese. The Km remains constant during these regulatory changes in Vmax. Manganese accumulated during growth is exchangeable for exogenous manganese and can be released from the cells by toluene (which causes leakage but not lysis) or by lysis with lysozyme. Two stages can be distinguished with regard to intracellular manganese during the process of growth and sporulation. During logarithmic growth, B. subtilis maintains a relatively constant internal manganese content, which is a function of the external manganese concentration following approximately a Langmuir adsorption isotherm. At the end of log phase, net accumulation of manganese slows. A second phase of net manganese accumulation begins at about the same time during sporulation as the accumulation of calcium begins. The manganese accumulated during growth and early sporulation is exchangeable and therefore relatively “free”; intracellular manganese is converted later during sporulation into a bound form that cannot be released by toluene or lysozyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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