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. 1973 Mar;113(3):1482–1490. doi: 10.1128/jb.113.3.1482-1490.1973

Mutants of Bacillus subtilis Temperature Sensitive in the Outgrowth Phase of Spore Germination

A Galizzi 1, F Gorrini 1, A Rollier 1, M Polsinelli 1
PMCID: PMC251720  PMID: 4632401

Abstract

Thirteen thermosensitive mutants of Bacillus subtilis defective in the outgrowth phase of spore germination were isolated. The spores of the mutants grow into vegetative cells at 35 C but not at 47 C, whereas the vegetative cells grow equally well at both temperatures. At 47 C all the mutant spores are able to initiate germination, but the process stops at an early phase of outgrowth in one strain and in a late phase in the other 12 strains. The spore of the latter gives rise to a swollen cell unable to divide. In all mutants, the normal phenotype is restored when the spores are grown in the presence of 20% sucrose or 2% NaCl. The synthesis of deoxyribonucleic acid and proteins does not seem to be altered in the mutants giving swollen cells. The mutants were grouped into three distinct genetic classes by transformation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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