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. 1973 Mar;113(3):1512–1514. doi: 10.1128/jb.113.3.1512-1514.1973

Fate of Transforming Deoxyribonucleic Acid After Uptake by Competent Bacillus subtilis: Nonrequirement of Deoxyribonucleic Acid Replication for Uptake and Integration of Transforming Deoxyribonucleic Acid

D Dubnau 1, C Cirigliano 1
PMCID: PMC251725  PMID: 4632402

Abstract

Studies on transformation of Bacillus subtilis using the inhibitor 6-(p-hydroxyphenylazo)-uracil show that deoxyribonucleic acid (DNA) replication is not required for the uptake and integration of donor DNA and genetic markers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodmer W. F. Integration of deoxyribonuclease-treated DNA in bacillus subtilis transformation. J Gen Physiol. 1966 Jul;49(6):233–258. doi: 10.1085/jgp.49.6.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bodmer W. F. Recombination and integration in Bacillus subtilis transformation: involvement of DNA synthesis. J Mol Biol. 1965 Dec;14(2):534–557. doi: 10.1016/s0022-2836(65)80203-0. [DOI] [PubMed] [Google Scholar]
  3. Brown N. C. Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis. J Mol Biol. 1971 Jul 14;59(1):1–16. doi: 10.1016/0022-2836(71)90409-8. [DOI] [PubMed] [Google Scholar]
  4. Cahn F. H., Fox M. S. Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients. J Bacteriol. 1968 Mar;95(3):867–875. doi: 10.1128/jb.95.3.867-875.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dooley D. C., Hadden C. T., Nester E. W. Macromolecular synthesis in Bacillus subtilis during development of the competent state. J Bacteriol. 1971 Nov;108(2):668–679. doi: 10.1128/jb.108.2.668-679.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubnau D., Cirigliano C. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA. J Mol Biol. 1972 Feb 28;64(1):9–29. doi: 10.1016/0022-2836(72)90318-x. [DOI] [PubMed] [Google Scholar]
  7. Dubnau D., Cirigliano C. Fate of transforming DNA following uptake by competent Bacillus subtilis. IV. The endwise attachment and uptake of transforming DNA. J Mol Biol. 1972 Feb 28;64(1):31–46. doi: 10.1016/0022-2836(72)90319-1. [DOI] [PubMed] [Google Scholar]
  8. Dubnau D., Davidoff-Abelson R. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex. J Mol Biol. 1971 Mar 14;56(2):209–221. doi: 10.1016/0022-2836(71)90460-8. [DOI] [PubMed] [Google Scholar]
  9. Erickson R. J., Braun W. Apparent dependence of transformation on the stage of deoxyribonucleic acid replication of recipient cells. Bacteriol Rev. 1968 Dec;32(4 Pt 1):291–296. [PMC free article] [PubMed] [Google Scholar]
  10. Erickson R. J., Copeland J. C. Structure and replication of chromosomes in competent cells of Bacillus subtilis. J Bacteriol. 1972 Mar;109(3):1075–1084. doi: 10.1128/jb.109.3.1075-1084.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hadden C., Nester E. W. Purification of competent cells in the Bacillus subtilis transformation system. J Bacteriol. 1968 Mar;95(3):876–885. doi: 10.1128/jb.95.3.876-885.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laird C. D., Wang L., Bodmer W. F. Recombination and DNA replication in Bacillus subtilis transformation. Mutat Res. 1968 Sep-Oct;6(2):205–209. doi: 10.1016/0027-5107(68)90035-3. [DOI] [PubMed] [Google Scholar]
  13. McCarthy C., Nester E. W. Macromolecular synthesis in newly transformed cells of Bacillus subtilis. J Bacteriol. 1967 Jul;94(1):131–140. doi: 10.1128/jb.94.1.131-140.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. VENEMA G., PRITCHARD R. H., VENEMA-SCHROEDER T. FATE OF TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS. J Bacteriol. 1965 May;89:1250–1255. doi: 10.1128/jb.89.5.1250-1255.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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