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. 1973 Apr;114(1):1–6. doi: 10.1128/jb.114.1.1-6.1973

Nuclear Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerases from Saccharomyces cerevisiae

Jesus Sebastian 1, Madhu M Bhargava 1, Harlyn O Halvorson 1
PMCID: PMC251732  PMID: 4572710

Abstract

Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA polymerase I has a higher affinity for polydeoxy-adenylic-thymidylic acid and RNA polymerase II for denatured DNA. RNA polymerase I is active only with manganese. α-Amanitin inhibits only the activity of RNA polymerase II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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