Abstract
The fractions of Escherichia coli female and male bacteria that are competent to form specific mating pairs at any time during normal exponential growth have been measured by a physical assay. The competencies of a Hayes- and a Cavali-type donor were 35 and 50%, respectively, in agreement with piliation assays reported by others. The competencies of two recipient strains, PA309 and P678, were found to be 33 and 29%, respectively.
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- ANDERSON T. F. Recombination and segregation in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1958;23:47–58. doi: 10.1101/sqb.1958.023.01.007. [DOI] [PubMed] [Google Scholar]
- Brinton C. C., Jr The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria. Trans N Y Acad Sci. 1965 Jun;27(8):1003–1054. doi: 10.1111/j.2164-0947.1965.tb02342.x. [DOI] [PubMed] [Google Scholar]
- CAVALLI-SFORZA L. L. La sessualità nei batteri. Boll Ist Sieroter Milan. 1950 Sep-Oct;29(9-10):281–289. [PubMed] [Google Scholar]
- Curtiss R., 3rd Bacterial conjugation. Annu Rev Microbiol. 1969;23:69–136. doi: 10.1146/annurev.mi.23.100169.000441. [DOI] [PubMed] [Google Scholar]
- Curtiss R., 3rd, Caro L. G., Allison D. P., Stallions D. R. Early stages of conjugation in Escherichia coli. J Bacteriol. 1969 Nov;100(2):1091–1104. doi: 10.1128/jb.100.2.1091-1104.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE ZWAIG R. N., ANTON D. N., PUIG J. The genetic control of colicinogenic factors E2, I and V. J Gen Microbiol. 1962 Nov;29:473–484. doi: 10.1099/00221287-29-3-473. [DOI] [PubMed] [Google Scholar]
- HAYES W. The mechanism of genetic recombination in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1953;18:75–93. doi: 10.1101/sqb.1953.018.01.016. [DOI] [PubMed] [Google Scholar]
- Ippen K. A., Valentine R. C. The sex hair of E. coli as sensory fiber, conjugation tube, or mating arm? Biochem Biophys Res Commun. 1967 Jun 23;27(6):674–680. doi: 10.1016/s0006-291x(67)80088-3. [DOI] [PubMed] [Google Scholar]
- Monner D. A., Jonsson S., Boman H. G. Ampicillin-resistant mutants of Escherichia coli K-12 with lipopolysaccharide alterations affecting mating ability and susceptibility to sex-specific bacteriophages. J Bacteriol. 1971 Aug;107(2):420–432. doi: 10.1128/jb.107.2.420-432.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mycielski R., Kociszewska-Kiljańczyk B., Kunicki-Goldfinger W. J. Mechanism of conjugation and recombination in bacteria. VI. Receptibility of F- cells for donor genophore during the cell-life cycle. Acta Microbiol Pol A. 1969;1(2):79–85. [PubMed] [Google Scholar]
- Novotny C., Knight W. S., Brinton C. C., Jr Inhibition of bacterial conjugation by ribonucleic acid and deoxyribonucleic acid male-specific bacteriophages. J Bacteriol. 1968 Feb;95(2):314–326. doi: 10.1128/jb.95.2.314-326.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ou J. T., Anderson T. F. Role of pili in bacterial conjugation. J Bacteriol. 1970 Jun;102(3):648–654. doi: 10.1128/jb.102.3.648-654.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spelina V., Stárka J. Kinetics of recombinant formation in synchronized cultures of Escherichia coli. J Bacteriol. 1968 Jun;95(6):2442–2443. doi: 10.1128/jb.95.6.2442-2443.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watanabe T., Arai T., Hattori T. Effects of cell wall polysaccharide on the mating ability of Salmonella typhimurium. Nature. 1970 Jan 3;225(5227):70–71. doi: 10.1038/225070a0. [DOI] [PubMed] [Google Scholar]