Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Apr;114(1):439–442. doi: 10.1128/jb.114.1.439-442.1973

Cryptic Plasmids in a Minicell-Producing Strain of Salmonella typhimurium

Ronald J Sheehy a,1, David P Allison a, Roy Curtiss III a,2
PMCID: PMC251783  PMID: 4572722

Abstract

A minicell-producing strain of Salmonella typhimurium contains two cryptic plasmids. One has a molecular weight of 2.6 × 106 to 2.8 × 106, is present in multiple copies per cell, and segregates into minicells. The other has a molecular weight of 130 × 106, is present in few copies per cell, and probably does not segregate into minicells.

Full text

PDF
439

Images in this article

441Image
on p.441

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ansz H. S., Zandberg J., van de Pol J. H., van Bruggen E. F. Circular DNA from Shigella paradysenteriae. Eur J Biochem. 1969 Jun;9(2):156–159. doi: 10.1111/j.1432-1033.1969.tb00589.x. [DOI] [PubMed] [Google Scholar]
  3. BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bazaral M., Helinski D. R. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513–3520. doi: 10.1021/bi00850a028. [DOI] [PubMed] [Google Scholar]
  5. CURTIS S. R., 3rd CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI. J Bacteriol. 1965 Jan;89:28–40. doi: 10.1128/jb.89.1.28-40.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carlton B. C., Helinski D. R. Heterogeneous circular DNA elements in vegetative cultures of Bacillus megaterium. Proc Natl Acad Sci U S A. 1969 Oct;64(2):592–599. doi: 10.1073/pnas.64.2.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carrier W. L., Setlow R. B. Paper strip method for assaying gradient fractions containing radioactive macromolecules. Anal Biochem. 1971 Oct;43(2):427–432. doi: 10.1016/0003-2697(71)90272-7. [DOI] [PubMed] [Google Scholar]
  8. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cozzarelli N. R., Kelly R. B., Kornberg A. A minute circular DNA from Escherichia coli 15. Proc Natl Acad Sci U S A. 1968 Jul;60(3):992–999. doi: 10.1073/pnas.60.3.992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Davis R. W., Davidson N. Electron-microscopic visualization of deletion mutations. Proc Natl Acad Sci U S A. 1968 May;60(1):243–250. doi: 10.1073/pnas.60.1.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dowman J. E., Meynell G. G. Pleiotropic effects of de-repressed bacterial sex factors on colicinogeny and cell wall structure. Mol Gen Genet. 1970;109(1):57–68. doi: 10.1007/BF00334046. [DOI] [PubMed] [Google Scholar]
  12. Freifelder D., Folkmanis A., Kirschner I. Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J Bacteriol. 1971 Mar;105(3):722–727. doi: 10.1128/jb.105.3.722-727.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ikeda H., Inuzuka M., Tomizawa J. I. P1-like plasmid in Escherichia coli 15. J Mol Biol. 1970 Jun 14;50(2):457–470. doi: 10.1016/0022-2836(70)90204-4. [DOI] [PubMed] [Google Scholar]
  14. KLEINSCHMIDT A. K., LANG D., JACHERTS D., ZAHN R. K. [Preparation and length measurements of the total desoxyribonucleic acid content of T2 bacteriophages]. Biochim Biophys Acta. 1962 Dec 31;61:857–864. [PubMed] [Google Scholar]
  15. Kline B. C. Inhibition of plasmid DNA replication by rifampin in Salmonella pullorum. Biochem Biophys Res Commun. 1972 Mar 24;46(6):2019–2025. doi: 10.1016/0006-291x(72)90753-x. [DOI] [PubMed] [Google Scholar]
  16. Lee C. S., Davidson N. Covalently closed minicircular DNA in Micrococcus lysodeikticus. Biochem Biophys Res Commun. 1968 Sep 6;32(5):757–762. doi: 10.1016/0006-291x(68)90304-5. [DOI] [PubMed] [Google Scholar]
  17. MACHATTIE L. A., BERNE K. I., THOMAS C. A., Jr ELECTRON MICROSCOPY OF DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:648–649. doi: 10.1016/s0022-2836(65)80019-5. [DOI] [PubMed] [Google Scholar]
  18. Olsen W. L., Schoenhard D. E. Demonstration of two cryptic plasmids in Salmonella pullorum MS53. J Bacteriol. 1972 May;110(2):786–788. doi: 10.1128/jb.110.2.786-788.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rush M. G., Gordon C. N., Warner R. C. Circular deoxyribonucleic acid from Shigella dysenteriae Y6R. J Bacteriol. 1969 Nov;100(2):803–808. doi: 10.1128/jb.100.2.803-808.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES