Abstract
A spontaneously arising regulatory mutant of the gluconate system in Escherichia coli was isolated. This mutant became constitutive, probably in one step, for gluconate high-affinity transport, gluconokinase, and gluconate-6-P dehydrase. The mutation involved (gntR18) is cotransducible with asd. Pseudorevertants, derived from a mutant (M2) that shows a long lag for growth on gluconate mineral medium, were also isolated and characterized. They give constitutive levels of gluconokinase and gluconate-6-P dehydrase but lack high-affinity transport function. Genetic experiments performed with one of these pseudorevertants (M4) indicate that it carries a secondary mutation in the gntR gene. The M4 phenotype is thus the result of the interaction of expression of a constitutive mutation (gntR4) with the mutation of strain M2 (gntM2).
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Nagel de Zwaig R., Zwaig N., Istúriz T., Sánchez R. S. Mutations affecting gluconate metabolism in Escherichia coli. J Bacteriol. 1973 May;114(2):463–468. doi: 10.1128/jb.114.2.463-468.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]