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. 1973 May;114(2):612–617. doi: 10.1128/jb.114.2.612-617.1973

Effects of Mutational Loss of Specific Intracellular Proteases on the Sporulation of Bacillus subtilis

James H Hageman a,1, Bruce C Carlton b,2
PMCID: PMC251816  PMID: 4196247

Abstract

Two protease-deficient mutants of Bacillus subtilis 168 (Trp) were isolated and compared with the parental strain with respect to production of intracellular proteases and sporulation. A mutant lacking the metal-requiring “neutral” protease intracellularly sporulated as well as the parental strain. A second mutant, deficient in an as yet uncharacterized intracellular protease, failed to sporulate normally. It is proposed that this new protease is also involved in intracellular protein turnover.

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Selected References

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  1. Balassa G. Biochemical genetics of bacterial sporulation. I. Unidirectional pleiotropic interactions among genes controlling sporulation in Bacillus subtilis. Mol Gen Genet. 1969;104(1):73–103. [PubMed] [Google Scholar]
  2. Boyer H. W., Carlton B. C. Production of two proteolytic enzymes by a transformable strain of Bacillus subtilis. Arch Biochem Biophys. 1968 Nov;128(2):442–455. doi: 10.1016/0003-9861(68)90050-7. [DOI] [PubMed] [Google Scholar]
  3. Deutscher M. P., Kornberg A. Biochemical studies of bacterial sporulation and germination. 8. Patterns of enzyme development during growth and sporulation of Baccillus subtilis. J Biol Chem. 1968 Sep 25;243(18):4653–4660. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Mandelstam J., Waites W. M. Sporulation in Bacillus subtilis. The role of exoprotease. Biochem J. 1968 Oct;109(5):793–801. doi: 10.1042/bj1090793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Michel J. F., Millet J. Physiological studies on early-blocked sporulation mutants of Bacillus subtilis. J Appl Bacteriol. 1970 Mar;33(1):220–227. doi: 10.1111/j.1365-2672.1970.tb05246.x. [DOI] [PubMed] [Google Scholar]
  7. Millet J., Acher R. Spécificité "hydrophobique" d'une endopeptidase isolée de Bacillus megaterium. Biochim Biophys Acta. 1968 Jan 8;151(1):302–305. doi: 10.1016/0005-2744(68)90194-0. [DOI] [PubMed] [Google Scholar]
  8. Millet J. Caractérisation de deux endopeptidases excrétées par B. subtilis Marburg au cours de la sporulation. Bull Soc Chim Biol (Paris) 1969 Jul 25;51(3):457–469. [PubMed] [Google Scholar]
  9. Millner S. N., Massie B. Detection of proteolytic enzymes after cellulose acetate electrophoresis. Anal Biochem. 1969 Oct 15;32(1):154–157. doi: 10.1016/0003-2697(69)90116-x. [DOI] [PubMed] [Google Scholar]
  10. Prestidge L., Gage V., Spizizen J. Protease activities during the course of sporulation on Bacillus subtilis. J Bacteriol. 1971 Sep;107(3):815–823. doi: 10.1128/jb.107.3.815-823.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schaeffer P. Asporogenous mutants of Bacillus subtilis Marburg. Folia Microbiol (Praha) 1967;12(3):291–296. doi: 10.1007/BF02868746. [DOI] [PubMed] [Google Scholar]
  12. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spudich J. A., Kornberg A. Biochemical studies of bacterial sporulation and germaination. VII. Protein turnover during sporulation of Bacillus subtilis. J Biol Chem. 1968 Sep 10;243(17):4600–4605. [PubMed] [Google Scholar]

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