Table 1.
The observed masses of the phosphopantetheinyl eliminated 1+ fragment ions.
| Protein Investigated | Pathway | Digestion method | Fragmentation Methodb | Ra | Elimination ion Aa (calculated) | Elimination ion Ba (calculated) |
|---|---|---|---|---|---|---|
| PigH(ACP1) | Prodigiosin | Trypsin | OCAD | A | 347.129(347.128) | ND |
| PigH(ACP1) | Prodigiosin | Trypsin | OCAD | B | 396.161(396.159) | ND |
| PigH-2ACP | Prodigiosin | not digested | IRMPD | A | 347.130(347.128) | ND |
| PigH-2ACP | Prodigiosin | not digested | IRMPD | B | 396.161(396.159) | ND |
| PigG | Prodigiosin | not digested | IRMPD | C | 354.152(354.149) | ND |
| PltL | Pyoluteorin | not digested | IRMPD | C | 354.156(354.149) | 452.132(452.126) |
| PltL | Pyoluteorin | not digested | IRMPD | D | 388.112(388.110) | ND |
| PltL | Pyoluteorin | not digested | IRMPD | E | 422.077(422.071) | 520.050(520.048) |
| PltL | Pyoluteorin | not digested | OCAD | F | 509.967(509.970) | ND |
| MycA (ACP2) | Mycosubtilin | Trypsin | OCAD | G | 345.151(345.148) | 443.124(443.125) |
| MycA (ACP2) | Mycosubtilin | Trypsin | OCAD | H | 346.180(346.180) | 444.154(444.157) |
| MycA (ACP2) | Mycosubtilin | Trypsin | OCAD | I | ND | 445.150(445.154) |
| MycA (PCP1) | Mycosubtilin | Trypsin | IRMPD | J | ND | 555.253(555.251) |
| MycA (PCP1) | Mycosubtilin | Trypsin | IRMPD | K | ND | 557.283(557.279) |
| MycA (PCP1) | Mycosubtilin | Trypsin | IRMPD | M | ND | 499.190(499.188) |
| MycA (PCP1) | Mycosubtilin | Trypsin | IRMPD | N | ND | 501.219(501.217) |
| MycA (PCP1) | Mycosubtilin | Trypsin | IRMPD | G | ND | 443.128(443.125) |
| MycA (PCP1) | Mycosubtilin | Trypsin | IRMPD | K | ND | 445.155(445.154) |
| AcpK | Orphan | not digested | IRMPD | G | 345.153(345.148) | 443.131(443.125) |
| AcpK | Orphan | not digested | IRMPD | N | ND | 401.119(499.201) |
| PksN | Orphan | Trypsin | IRMPD | O | ND | 446.143(446.136) |
| PksJ (A1) | Orphan | Trypsin | IRMPD | P | ND | 477.150(477.146) |
| PksL-2ACP | Orphan | not digested | IRMPD | G | 345.151(345.148) | 443.127(443.125)c |
| PksL-2ACP | Orphan | not digested | IRMPD | Q | 405.170(405.170) | 503.149(503.146)c |
| PksL-2ACP | Orphan | not digested | IRMPD | R | 343.171(343.170) | 441.147(441.146)c |
| PksL-2ACP | Orphan | not digested | IRMPD | S | 407.173(407.174) | ND |
| PksL-2ACP | Orphan | not digested | IRMPD | T | 406.176(407.176) | ND |
| PksL-2ACP | Orphan | not digested | IRMPD | U | 387.162(387.159) | 485.139(485.136) |
| NikP1 | Nikkomycin | CNBr | OCAD | V | ND | 496.164(496.163) |
| NikP1 | Nikkomycin | not digested | IRMPD | V | ND | 496.163(496.163) |
| EntB | Enterobactin | not digested | IRMPD | W | ND | 495.118(495.120) |
| GrsA | Gramicidin | not digested | IRMPD | X | 408.198(408.196) | 506.179(506.173) |
| GrsA | Gramicidin | not digested | IRMPD | Y | 413.231(413.227) | 511.212(511.201) |
a
The structures representing R on the elimination ions are shown in figure 5.
b
Structures of Pant and PPant are shown in figure 4 as well.
c
Values taken from reference 19. ND = Not detected