FIG. 3.

Downregulation of p57 in cultured human islet cells and eGFP⁺ cells derived from β-cells. A: qPCR analysis of RNA extracted from islet cells derived from eight donors at the indicated passage numbers. P indicates passage number and weeks in culture. RQ indicates relative quantification compared with P0. Data are means ± SD (n = 8). Stars indicate statistical significance, compared with P0 (P < 0.03). B and C: Immunofluorescence analysis of eGFP⁺ cells derived from β-cells following 10 days in culture. Solid arrow in B points to an eGFP⁺ cell that has lost both insulin and p57 expression; dashed arrow points to an eGFP⁺ β-cell that maintains both insulin and p57 expression. Arrow in C points to an eGFP⁺ cell that maintains p57 expression and is not labeled for Ki67. Bar = 20 μm. (Please see http://dx.doi.org/10.2337/db07-1323 for a high-quality digital representation of this figure.)