Abstract
Mutations in capR or capT cause derepression of the enzymes of the gal operon. The gal-specific messenger ribonucleic acid is directly proportional to the gal enzyme levels in wild type, capR, and capT strains. These results indicate that capR and capT control the gal operon at the transcriptional level.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. II. LE D'ETERMINISME G'EN'ETIQUE DE LA R'EGULATION. J Mol Biol. 1963 Aug;7:183–205. doi: 10.1016/s0022-2836(63)80045-5. [DOI] [PubMed] [Google Scholar]
- Feiss M., Adyha S., Court D. L. Isolation of plaque-forming, galactose-transducing strains of phage lambda. Genetics. 1972 Jun;71(2):189–206. doi: 10.1093/genetics/71.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
- Guha A., Tabaczyński M., Szybalski W. Orientation of transcription for the galactose operon as determined by hybridization of gal mRNA with the separated DNA strands of coliphages lambda-dg. J Mol Biol. 1968 Jul 14;35(1):207–213. doi: 10.1016/s0022-2836(68)80048-8. [DOI] [PubMed] [Google Scholar]
- Hua S. S., Markovitz A. Multiple regulator gene control of the galactose operon in Escherichia coli K-12. J Bacteriol. 1972 Jun;110(3):1089–1099. doi: 10.1128/jb.110.3.1089-1099.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kalckar H. M., Kurahashi K., Jordan E. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1776–1786. doi: 10.1073/pnas.45.12.1776. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mackie G., Wilson D. B. Regulation of the gal operon of Escherichia coli by the capR gene. J Biol Chem. 1972 May 25;247(10):2973–2978. [PubMed] [Google Scholar]
- Miller Z., Varmus H. E., Parks J. S., Perlman R. L., Pastan I. Regulation of gal messenger ribonucleic acid synthesis in Escherichia coli by 3',5'-cyclic adenosine monophosphate. J Biol Chem. 1971 May 10;246(9):2898–2903. [PubMed] [Google Scholar]
- OKAMOTO K., SUGINO Y., NOMURA M. Synthesis and turnover of phage messenger RNA in E. coli infected with bacteriophage T4 in the presence of chloromycetin. J Mol Biol. 1962 Nov;5:527–534. doi: 10.1016/s0022-2836(62)80126-0. [DOI] [PubMed] [Google Scholar]
- Parks J. S., Gottesman M., Shimada K., Weisberg R. A., Perlman R. L., Pastan I. Isolation of the gal repressor. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1891–1895. doi: 10.1073/pnas.68.8.1891. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saedler H., Gullon A., Fiethen L., Starlinger P. Negative control of the galactose operon in E. coli. Mol Gen Genet. 1968;102(1):79–88. doi: 10.1007/BF00341872. [DOI] [PubMed] [Google Scholar]
- Shapiro J. A., Adhya S. L. The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity. Genetics. 1969 Jun;62(2):249–264. doi: 10.1093/genetics/62.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Varmus H. E., Perlman R. L., Pastan I. Regulation of lac messenger ribonucleic acid synthesis by cyclic adenosine 3',5'-monophosphate and glucose. J Biol Chem. 1970 May 10;245(9):2259–2267. [PubMed] [Google Scholar]