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. Author manuscript; available in PMC: 2009 Jul 4.
Published in final edited form as: Vaccine. 2008 May 20;26(29-30):3587–3593. doi: 10.1016/j.vaccine.2008.04.079

Table 1.

Competitive inhibition ELISA. Plates were coated with B. bronchiseptica 10580, Rb50 or B. parapertussis 15989 LPS at 5 ug/ml and reacted with anti-B. bronchiseptica 10580, anti-Rb50 or anti-B. parapertussis 15989 hyperimmune mice sera. Inhibitors were used at 50 ug/well.

Inhibitor O-SP end-group Amidation of 2nd O-SP sugar % of inhibition of hyperimmune whole cell sera
anti-Bb10580 anti-BbRb50 anti-Bpp15989
Bb 10580 O-SP Ala 92 2 50
Bb 110H O-SP Ala 96 3 45
Bb Rb50 O-SP Lac 0 93 1
Bb 512 O-SP Lac 1 95 3
Bpp 15989 O-SP Ala + 42 5 97
Bpp 15311 O-SP Ala + 50 3 98
H. ducreyi O-SP na1 na 0 0 0
1

na - not applicable