Abstract
Gramicidin and valinomycin in concentrations of 10−7 and 10−6m, respectively, inhibited the growth of Streptococcus faecalis. Inhibition of growth was associated with loss of Rb+ and K+ from the cells, and could be reversed by addition of excess K+. Cells treated with these antibiotics exhibited greatly increased permeability to certain cations; no effect was observed on the penetration of other small molecules. Unlike normal cells, cells treated with gramicidin rapidly lost internal Rb+ by passive exchange with external cations, including H+, all monovalent alkali metals, NH4+, Mg++, and tris(hydroxymethyl)aminomethane. Exchange was rapid even at 0 C and was independent of energy metabolism. The effect of valinomycin was more selective. Cellular Rb+ was rapidly displaced by external H+, K+, Rb+, and Cs+; other cations were less effective. The exchange was independent of metabolism but strongly affected by temperature. Under certain conditions, polyvalent cations inhibited exchange between 86Rb and Rb+ induced by valinomycin. The antibiotic apparently neither stimulates nor inhibits the energy-dependent K+ pump of S. faecalis, but exerts its effect on the passive permeability of the membrane to cations. The increased permeability to specific cations induced by gramicidin and valinomycin is a sufficient explanation for the inhibition of growth, glycolysis, and other processes.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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