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. 1967 Jul;94(1):75–79. doi: 10.1128/jb.94.1.75-79.1967

Cellular Sites for the Competence-provoking Factor of Streptococci

Roman Pakula 1
PMCID: PMC251873  PMID: 6028002

Abstract

Immune globulins against competent cells of group H streptococci, strains Challis and Wicky, inhibited genetic transformation to streptomycin resistance when added to competent cultures. Antibodies against noncompetent cells did not inhibit transformation of competent cells. Strain Challis is spontaneously highly transformable. Strain Wicky is very poorly transformable but can be converted to high transformability with the exocellular competence-provoking factor (CPF) produced by strain Challis. Globulins against noncompetent cells of strain Challis and Wicky also inhibited transformation when added to noncompetent cultures prior to conversion to competence. Antibodies against cells of the related strain Blackburn, however, did not inhibit transformation under any circumstances. It is concluded that, although globulins prepared against competent cells block the deoxyribonucleic acid receptor sites present in these cells, the globulins prepared against noncompetent cells prevent conversion to competence by blocking the access of CPF to specific cellular sites for this factor. Strain Blackburn seems not to contain CPF-receptive sites and is, therefore, nontransformable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colman G., Williams R. E. The cell walls of streptococci. J Gen Microbiol. 1965 Dec;41(3):375–387. doi: 10.1099/00221287-41-3-375. [DOI] [PubMed] [Google Scholar]
  2. NASTUK W. L., PLESCIA O. J., OSSERMAN K. E. Changes in serum complement activity in patients with myasthenia gravis. Proc Soc Exp Biol Med. 1960 Oct;105:177–184. doi: 10.3181/00379727-105-26050. [DOI] [PubMed] [Google Scholar]
  3. NAVA G. C., GALIS A., BEISER S. M. Bacterial transformation: an antigen specific for 'competent' pneumococci. Nature. 1963 Mar 2;197:903–904. doi: 10.1038/197903b0. [DOI] [PubMed] [Google Scholar]
  4. Osowiecki H., Lancow W. A. Incorporation of 32P-labelled DNA by some strains of group H streptococci in the presence of albumin or the transformation-provoking factor. Bull Acad Pol Sci Biol. 1965;13(6):339–343. [PubMed] [Google Scholar]
  5. PAKULA R., CYBULSKA J., WALCZAK W. THE EFFECT OF ENVIRONMENTAL FACTORS ON TRANSFORMABILITY OF A STREPTOCOCCUS. Acta Microbiol Pol. 1963;12:245–257. [PubMed] [Google Scholar]
  6. PAKULA R., WALCZAK W. On the nature of competence of transformable streptococci. J Gen Microbiol. 1963 Apr;31:125–133. doi: 10.1099/00221287-31-1-125. [DOI] [PubMed] [Google Scholar]
  7. PAKULA R., WALCZAK W., SHUGAR D. Oxygen and dose-rate effects on survival curves of gamma-irradiated transforming DNA in the presence of protective substances. Acta Biochim Pol. 1962;9:227–237. [PubMed] [Google Scholar]
  8. Pakula R., Hauschild A. H. The effect of "competase" on DNA uptake in provoked transformation of a streptococcus. Can J Microbiol. 1965 Oct;11(5):823–827. doi: 10.1139/m65-111. [DOI] [PubMed] [Google Scholar]
  9. Pakula R. Inhibition of transformation of streptococci by antibodies. J Bacteriol. 1965 Nov;90(5):1501–1501. doi: 10.1128/jb.90.5.1501-.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pakula R. Production of competence-provoking factor and development of competence of a transformable streptococcus in serum-free media. Can J Microbiol. 1965 Oct;11(5):811–822. doi: 10.1139/m65-110. [DOI] [PubMed] [Google Scholar]
  11. Spencer H. T., Herriott R. M. Development of competence of Haemophilus influenzae. J Bacteriol. 1965 Oct;90(4):911–920. doi: 10.1128/jb.90.4.911-920.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. THOMAS R. Recherches sur la cinétique des transformations bactériennes. Biochim Biophys Acta. 1955 Dec;18(4):467–481. doi: 10.1016/0006-3002(55)90137-2. [DOI] [PubMed] [Google Scholar]
  13. Tomasz A., Beiser S. M. Relationship between the competence antigen and the competence-activator substance in pneumococci. J Bacteriol. 1965 Nov;90(5):1226–1232. doi: 10.1128/jb.90.5.1226-1232.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tomasz A. Model for the mechanism controlling the expression of competent state in Pneumococcus cultures. J Bacteriol. 1966 Mar;91(3):1050–1061. doi: 10.1128/jb.91.3.1050-1061.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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