Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Jul;94(1):196–201. doi: 10.1128/jb.94.1.196-201.1967

Ultrastructure of the Obligate Halophilic Bacterium Halobacterium halobium

K Y Cho 1, Colin H Doy 1, E H Mercer 1
PMCID: PMC251890  PMID: 6027988

Abstract

The fine structure of Halobacterium halobium was studied by means of a modified double-fixation technique. The cell envelope is shown to consist of both a “wall” and a plasma membrane. Some electron-dense strands were seen inside the cytoplasm running parallel to the cell envelope. An unusual organelle (or organelles) appeared inside the cytoplasm in the form of parallel striated strands.

Full text

PDF
196

Images in this article

198Image
on p.198
199Image
on p.199
200Image
on p.200

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN A. D., SHOREY C. D. THE CELL ENVELOPES OF TWO EXTREMELY HALOPHILIC BACTERIA. J Cell Biol. 1963 Sep;18:681–689. doi: 10.1083/jcb.18.3.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHAPMAN G. B., HILLIER J. Electron microscopy of ultra-thin sections of bacteria I. Cellular division in Bacillus cereus. J Bacteriol. 1953 Sep;66(3):362–373. doi: 10.1128/jb.66.3.362-373.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cho K. Y., Salton M. R. Fatty acid composition of bacterial membrane and wall lipids. Biochim Biophys Acta. 1966 Feb 1;116(1):73–79. doi: 10.1016/0005-2760(66)90093-2. [DOI] [PubMed] [Google Scholar]
  4. DEPETRIS S. ULTRASTRUCTURE OF THE CELL WALL OF ESCHERICHIA COLI. J Ultrastruct Res. 1965 Apr;12:247–262. doi: 10.1016/s0022-5320(65)80098-3. [DOI] [PubMed] [Google Scholar]
  5. FITZ-JAMES P. C. Participation of the cytoplasmic membrane in the growth and spore fromation of bacilli. J Biophys Biochem Cytol. 1960 Oct;8:507–528. doi: 10.1083/jcb.8.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GLAUERT A. M. The fine structure of bacteria. Br Med Bull. 1962 Sep;18:245–250. doi: 10.1093/oxfordjournals.bmb.a069988. [DOI] [PubMed] [Google Scholar]
  7. HOUWINK A. L. Flagella, gas vacuoles and cell-wall structure in Halobacterium halobium; an electron microscope study. J Gen Microbiol. 1956 Aug;15(1):146–150. doi: 10.1099/00221287-15-1-146. [DOI] [PubMed] [Google Scholar]
  8. IMAEDA T., OGURA M. Formation of intracytoplasmic membrane system of mycobacteria related to cell division. J Bacteriol. 1963 Jan;85:150–163. doi: 10.1128/jb.85.1.150-163.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MURRAY R. G., STEED P., ELSON H. E. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. Can J Microbiol. 1965 Jun;11:547–560. doi: 10.1139/m65-072. [DOI] [PubMed] [Google Scholar]
  10. MURRAY R. G., WATSON S. W. STRUCTURE OF NITROSOCYSTIS OCEANUS AND COMPARISON WITH NITROSOMONAS AND NITROBACTER. J Bacteriol. 1965 Jun;89:1594–1609. doi: 10.1128/jb.89.6.1594-1609.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. OHYE D. F., MURRELL W. G. Formation and structure of the spore of Bacillus coagulans. J Cell Biol. 1962 Jul;14:111–123. doi: 10.1083/jcb.14.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pangborn J., Marr A. G., Robrish S. A. LOCALIZATION OF RESPIRATORY ENZYMES IN INTRACYTOPLASMIC MEMBRANES OF AZOTOBACTER AGILIS. J Bacteriol. 1962 Oct;84(4):669–678. doi: 10.1128/jb.84.4.669-678.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RYTER A., JACOB F. ETUDE AU MICROSCOPE 'ELECTRONIQUE DES RELATIONS ENTRE M'ESOSOMES ET NOYAUX CHEZ BACILLUS SUBTILIS. C R Hebd Seances Acad Sci. 1963 Nov 13;257:3060–3063. [PubMed] [Google Scholar]
  15. Sedar A. W., Burde R. M. The demonstration of the succinic dehydrogenase system in Bacillus subtilis using tetranitro--blue tetrazolium combined with techniques of electron microscopy. J Cell Biol. 1965 Oct;27(1):53–66. doi: 10.1083/jcb.27.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. VAN ITERSON, LEENE W. A CYTOCHEMICAL LOCALIZATION OF REDUCTIVE SITES IN A GRAM-POSITIVE BACTERIUM. TELLURITE REDUCTION IN BACILLUS SUBTILIS. J Cell Biol. 1964 Mar;20:361–375. doi: 10.1083/jcb.20.3.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. VAN ITERSON W. Some features of a remarkable organelle in Bacillus subtilis. J Biophys Biochem Cytol. 1961 Jan;9:183–192. doi: 10.1083/jcb.9.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES