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. Author manuscript; available in PMC: 2008 Aug 25.
Published in final edited form as: Biochemistry. 2006 Oct 3;45(39):11799–11808. doi: 10.1021/bi060600w

Figure 2. Formation of reconstituted particles with DMPC among the domains in B17.

Figure 2

Chromatograms of three individual domains in B17 incubated with DMPC MLVs: the β-barrel (a), α-helical (b) and C-sheet (c). Proteins were prepared in TS buffer with (black) or without (light gray) DMPC MLVs at 1:1 (wt:wt) ratio and incubated at 24 °C for 16 hours. The β-barrel and α-helical domains were prepared at 1 mg/ml, whereas the C-sheet was prepared at 0.2 mg/ml. The protein/lipid mixtures after incubation were centrifuged and then analyzed by a superdex GL200 column at 4 °C.