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. 1967 Sep;94(3):700–706. doi: 10.1128/jb.94.3.700-706.1967

Effect of Rec− Mutations on the Activity of Colicinogenic Factors

Donald R Helinski 1, Harvey R Herschman 1
PMCID: PMC251941  PMID: 5340681

Abstract

The effect of two Rec− mutations (AB2463 and JC1553) on the ability of a cell to accept, maintain, and express the colicinogenic factors ColE1, ColE2, and ColV was examined in Escherichia coli. These mutations had no observable effect on the colicinogenic properties of the ColV factor, but prevented the spontaneous and induced production of colicins E1 and E2 which are determined by the ColE1 and ColE2 factors, respectively. The two Rec− mutations had no apparent effect on the ability of the cells to acquire, maintain, or transfer the ColE1 and ColE2 factors. These mutations did not affect the expression of immunity by any of the three Col factors. ColE1 and ColE2 were also shown to be indirectly induced by mating F− cells carrying these Col factors with ultraviolet-irradiated, non-colicinogenic, Hfr and F+ cells. Indirect induction of colicin production occurred when either an irradiated F+ Rec+ or F+ Rec− strain was employed as the donor strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borek E., Ryan A. THE TRANSFER OF IRRADIATION-ELICITED INDUCTION IN A LYSOGENIC ORGANISM. Proc Natl Acad Sci U S A. 1958 May;44(5):374–377. doi: 10.1073/pnas.44.5.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks K., Clark A. J. Behavior of lambda bacteriophage in a recombination deficienct strain of Escherichia coli. J Virol. 1967 Apr;1(2):283–293. doi: 10.1128/jvi.1.2.283-293.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CLOWES R. C. Colicine factors as fertility factors in bacteria: Escherichia coli K-12. Nature. 1961 Jun 10;190:988–989. doi: 10.1038/190988a0. [DOI] [PubMed] [Google Scholar]
  5. FREDERICQ P. Colicins. Annu Rev Microbiol. 1957;11:7–22. doi: 10.1146/annurev.mi.11.100157.000255. [DOI] [PubMed] [Google Scholar]
  6. Fuerst C. R., Siminovitch L. Characterization of an unusual defective lysogenic strain of Escherichia coli K-12(lambda). Virology. 1965 Nov;27(3):449–451. doi: 10.1016/0042-6822(65)90131-5. [DOI] [PubMed] [Google Scholar]
  7. GOLDTHWAIT D., JACOB F. SUR LE M'ECANISME DE L'INDUCTION DU D'EVELOPPEMENT DU PROPHAGE CHEZ LES BACT'ERIES LYSOG'ENES. C R Hebd Seances Acad Sci. 1964 Jul 20;259:661–664. [PubMed] [Google Scholar]
  8. Herschman H. R., Helinski D. R. Comparative study of the events associated with colicin induction. J Bacteriol. 1967 Sep;94(3):691–699. doi: 10.1128/jb.94.3.691-699.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hertman I., Luria S. E. Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda. J Mol Biol. 1967 Jan 28;23(2):117–133. doi: 10.1016/s0022-2836(67)80021-4. [DOI] [PubMed] [Google Scholar]
  10. Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KAHN P., HELINSKI D. R. RELATIONSHIP BETWEEN COLICINOGENIC FACTORS E1 AND V AND AN F FACTOR IN ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1573–1579. doi: 10.1128/jb.88.6.1573-1579.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kahn P. L., Helinski D. R. Interaction between colicinogenic factor V and the integrated F factor in an Hfr strain of Escherichia coli. J Bacteriol. 1965 Nov;90(5):1276–1282. doi: 10.1128/jb.90.5.1276-1282.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NAGELDEZWAIG R., PUIG J. THE GENETIC BEHAVIOUR OF COLICINOGENIC FACTOR E. J Gen Microbiol. 1964 Aug;36:311–321. doi: 10.1099/00221287-36-2-311. [DOI] [PubMed] [Google Scholar]
  14. OZEKI H., STOCKER B. A., DE MARGERIE H. Production of colicine by single bacteria. Nature. 1959 Aug 1;184:337–339. doi: 10.1038/184337a0. [DOI] [PubMed] [Google Scholar]
  15. SMITH S. M., OZEKI H., STOCKER B. A. TRANSFER OF COLE1 AND COLE2 DURING HIGH-FREQUENCY TRANSMISSION OF COLI IN SALMONELLA TYPHIMURIUM. J Gen Microbiol. 1963 Nov;33:231–242. doi: 10.1099/00221287-33-2-231. [DOI] [PubMed] [Google Scholar]

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