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. 1967 Sep;94(3):759–765. doi: 10.1128/jb.94.3.759-765.1967

Comparisons of Cells, Refractile Bodies, and Spores of Bacillus popilliae1

Brij M Mitruka 1,2,2, Ralph N Costilow 1,2, S H Black 1,2, R E Pepper 1,2
PMCID: PMC251948  PMID: 6035269

Abstract

Spores of Bacillus popilliae from infected larvae and refractile bodies produced in a Trypticase-barbiturate medium were similar but distinct from vegetative cells of this organism in protein, nucleic acid, and enzyme composition. The spores and refractile bodies were found to have catalase activity, some of which was heat-resistant. This enzyme was not found in the vegetative cells. The spores contained dipicolinic acid, but the refractile bodies did not. The latter were similar to cells in having considerably higher levels of phosphate extractable with cold trichloroacetic acid and of poly-β-hydroxybutyrate than had the spores. Electron microscopy demonstrated conclusively that the refractile bodies are distinctly different from either cells or spores of B. popilliae. The possibility that these bodies are formed as a result of an aborted sporulation process is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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