Volume 188, no. 20, p. 7222-7229, 2006. Page 7225, Figure 1B: The data in this figure are correct. However, the ΔcseA panel was previously published in error, labeled “ΔsigE,” in reference 1, and the ΔsigE panel has therefore never been published. To clarify, the complete data set, originally split between the two papers, is reproduced here. A similar correction will appear in Molecular Microbiology (2).
FIG. 1.
Induction of the sigE promoter (sigEp) in its native chromosomal context by vancomycin. S1 nuclease protection analysis of the sigE transcript in S. coelicolor M600 (wt), J2130 (ΔsigE), J2172 (ΔcseA), and J2142 (ΔcseB::hyg) grown to mid- to late exponential phase in NMMP liquid medium containing 2 mM MgCl2 and exposed to 10 μg/ml vancomycin. RNA was extracted from samples taken immediately prior to the addition of vancomycin and at subsequent 30-min intervals. Relative signal strengths were quantified on a Phosphorimager; each signal is expressed as a percentage of the strongest signal.
References
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