Abstract
A generally accepted view of norovirus replication is that capsid expression requires production of a subgenomic transcript, the presence of capsid often being used as a surrogate marker to indicate the occurrence of viral replication. Using a polymerase II-based baculovirus delivery system, we observed capsid expression following introduction of a full-length genogroup 3 norovirus genome into HepG2 cells. However, capsid expression occurred as a result of a novel translation termination/reinitiation event between the nonstructural-protein and capsid open reading frames, a feature that may be unique to genogroup 3 noroviruses.
Noroviruses are nonenveloped, positive-strand RNA viruses belonging to the family Caliciviridae and are the single most frequent cause of human nonbacterial gastroenteritis worldwide (6). Their highly infectious nature means that outbreaks are difficult to contain. No vaccine exists for noroviruses, and although there is a cell culture system for mouse norovirus (MNV) (17), routine propagation of enteron-specific noroviruses has yet to be achieved. Indeed, information regarding norovirus replication has mostly been obtained by drawing parallels with the more distantly related but cultivatable pathogen feline calicivirus (1).
Caliciviruses possess certain features that distinguish them from other positive-strand RNA viruses. The 5′ end of their genome is covalently linked to a viral protein (VPg), which both serves as a primer for genome replication (13) and directs translation of viral proteins (2, 3, 5). The 3′ end contains a short untranslated region as well as a poly(A) tail. Several open reading frames (ORFs) exist within a calicivirus genome. In noroviruses, there are three verified ORFs, with the largest of these (ORF1) being translated from the viral genomic RNA and encoding nonstructural proteins (Fig. 1a). Translation of the major structural protein, encoded by ORF2, is currently believed to be dependent on production of a subgenomic RNA, which also allows for ORF3 expression due to translation termination reinitiation (TTR) that occurs between ORF2 and ORF3 (4, 11, 16).
We recently developed a baculovirus-based delivery system for recovery of infectious MNV (15). A parallel attempt to develop a reverse genetics system for bovine genogroup 3 noroviruses, which are more closely related to human noroviruses (7), is in progress. As a result of this work, we now report a novel observation, that bovine norovirus can express capsid protein as a result of TTR between ORF1 and ORF2.
Characterization of cells transduced with genogroup 3-containing baculovirus.
A cDNA of a genotype 3 norovirus genome, derived from the Jena virus (JV) (7), was cloned into the polymerase II-based baculovirus delivery system (9, 10, 15) (Fig. 1b). Recovered baculovirus, referred to as JV(wt), was used to transduce HepG2 cells in combination with BACtTA, a construct expressing the tetracycline repressor/VP16 transactivator. At 20 hours posttransduction, fully processed and proteolytic precursors of VPg and 3C were detected by Western blot analysis (Fig. 2a, lane 2), confirming ORF1 expression in these cells. To assess whether viral replication might be occurring, Western blotting was used to detect capsid expression from ORF2. As work with the related MNV reverse genetics system had failed to demonstrate capsid expression, despite recovery of infectious virus (15), it was surprising that JV capsid expression was detectable (Fig. 2a, lane 2). While the capsid monoclonal antibody used cross-reacted with other cellular antigens of various molecular weights, detection of capsid was nonetheless specific, as it was also observed using a rabbit anti-capsid antiserum and was absent when cells were maintained in the presence of tetracycline (data not shown).
Capsid expression indicated that viral replication might be occurring; therefore, cellular RNA was analyzed by Northern blotting for the presence of a subgenomic transcript. While a major single transcript of the expected size for the full-length genome was present, no subgenomic RNA production was observed (Fig. 2b, lane 2).
Generation of replication-defective constructs where capsid expression is maintained.
To establish further whether low-level viral replication and subgenomic RNA production were the cause of capsid expression, two different replication-defective JV constructs were generated (Fig. 1a). The first construct, JV(ΔPol), had a frameshift mutant at the 3′ end of ORF1 so that premature translational termination occurred within the 3D viral polymerase-encoding region. The second construct, JV(GND), was mutated at the GDD active site within 3D to inactivate polymerase activity (14). Both constructs expressed ORF1 products, based on Western blot analysis (Fig. 2a, lanes 3 and 4), and showed proteolytic processing of ORF1 similar to that shown by the parental JV construct, albeit with the expected generation of some shorter precursor products in JV(ΔPol) transduced cells. However, while capsid was not expressed from JV(ΔPol), it was readily detectable in cells transduced with JV(GND), demonstrating that the presence of capsid was not due to viral replication. Furthermore, Northern blot analysis (Fig. 2b, lanes 3 and 4) showed that both constructs resulted in the expression of a single major transcript that was identical in size to that produced from JV(wt), indicating that the changes introduced into the JV genome had not facilitated unanticipated RNA splicing that might account for differential capsid expression.
Identification of a functional TURBS1 motif at the 3′ end of ORF1.
One explanation that would link capsid expression with a requirement for translation of an intact ORF1 is that JV employs a TTR mechanism at the ORF1/2 boundary: a mechanism that was thought to be restricted to the ORF2/3 boundary in noroviruses. Two RNA elements at the end of ORF2 that allow TTR to occur have recently been described (8, 12). They have been named translational upstream ribosome binding sites (TURBS), and the presence of both is essential for TTR. While the sequence of the second TURBS is highly variable, preventing identification based on sequence analysis, the sequence of the first TURBS, which is typically found approximately 30 to 60 nucleotides upstream of ORF3, has a conserved motif that is speculated to base pair with the 18S ribosomal subunit. Interestingly, examination of the nucleotide sequence at the end of ORF1 of JV identified a putative TURBS motif that was appropriately positioned with respect to the start of ORF2 (Fig. 3a). Therefore, a further Jena construct, JV(TURBSko), was generated; in this construct, the TURBS motif was disrupted through introduction of a conservative, nonsynonymous mutation within ORF1 (Fig. 1a and 3b). Transduction of cells with this construct resulted in expression of an intact ORF1 locus, based on Western blot analysis (Fig. 2a, lane 5). However, the mutation introduced into the TURBS1 motif resulted in a loss of capsid expression (Fig. 2a, lane 5), indicating that capsid expression was due to translational termination/reinitiation.
To further substantiate this hypothesis, additional T7-based constructs were generated. These contained both Renilla and firefly luciferase sequences separated by a JV ORF1/2 encoding region such that the two luciferases were expressed in-frame with ORF1 and ORF2, respectively, and served as a marker for ORF translation (Fig. 4a). When transfected into cells as capped RNA transcripts, both Renilla and firefly luciferase activities could be detected (Fig. 4b). However, when the same mutation used to disrupt the TURBS1 motif in JV(TURBko) was present, firefly activity relative to Renilla activity was reduced by ∼95%, reconfirming the importance of this motif for ORF2 expression. More importantly, when cells were transfected with noncapped versions of the same RNAs, the activities of both Renilla and firefly luciferases fell by equivalent amounts, demonstrating that ORF2 expression is directly dependent on cap-dependent translation of ORF1.
Characterization of TTR between ORF1 and ORF2.
The characteristic features of previously characterized TTR events in caliciviruses are that they require a correctly positioned stop codon in the upstream ORF but that the AUG start codon in the downstream ORF is at least partially dispensable (8, 11). To investigate whether the TTR event between JV ORF1 and ORF2 behaved in a comparable fashion, a series of mutations were introduced into the above-described luciferase-based construct (Fig. 4a). Disruption of the ORF1 stop codon by a single nucleotide change to generate a Trp codon (lane Opal>Trp), which extended ORF1 by a further 38 codons, reduced firefly expression by 95% (Fig. 4c). However, this was restored by an additional nucleotide change that reintroduced the stop codon at the end of ORF1 (lane Opal>Amber) or by an exchange of the original stop codon for an Ochre stop codon (lane Opal>Ochre), demonstrating that the location of this stop codon is indeed important for ORF2 expression. In contrast, the two potential AUG start codons in ORF2 were less influential in determining whether firefly expression occurred. Disruption of either of them individually did not affect firefly expression, and when both were disrupted, a significant albeit reduced level of ORF2 translation was still observed, confirming that AUG-independent translation initiation indeed contributes to ORF2 protein production. To exclude the possibility that this residual expression was due to AUG-dependent initiation elsewhere, a stop codon was introduced at the end of the ORF2 sequence (lane 3′stop). Firefly expression from this construct was effectively reduced to levels comparable to that of the TURBSko construct, demonstrating that AUG-independent translation initiation was occurring. Therefore, the features of the TTR event at the JV ORF1/2 boundary are essentially identical to those of other caliciviruses.
In summary, the experimental results demonstrate that TTR is an additional novel mechanism that genogroup 3 noroviruses use to express capsid. While sequence analysis suggests that a putative TURBS1 motif might also exist at the end of ORF1 for other genogroups (Fig. 3a), experimental data suggest the opposite, at least for genogroup 2 viruses (see Fig. S1 in the supplemental material). Possible advantages that might be gained from the use of TTR by genogroup 3 noroviruses are unclear, as translation of ORF2 from the viral genome is unlikely to make a significant contribution to overall levels of capsid expression in an infected cell since expression of the same protein from subgenomic RNA transcripts is likely to be more effective. However, other genera of the Caliciviridae family, comprising sapoviruses and lagoviruses, also achieve capsid expression independently of subgenomic RNA production by having the nonstructural-protein- and capsid-encoding regions contained within the same ORF. Therefore, it is tempting to speculate that in some caliciviruses, capsid plays a role beyond that of a structural protein, which is critical for earlier steps in the virus life cycle occurring before subgenomic RNA production, and the existence of TTR between ORF1 and ORF2 in genogroup 3 noroviruses reflects this.
Supplementary Material
Acknowledgments
This work was supported by a pump-priming fund provided by Southampton School of Medicine to C.J.M. and by Welcome Trust Grant 069233.
Footnotes
Published ahead of print on 25 June 2008.
Supplemental material for this article may be found at http://jvi.asm.org/.
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