Abstract
The rare earth cerium was found to bind rapidly to Escherichia coli. Cerium inhibited oxygen uptake in the presence of glucose as well as the endogenous respiration of glucose-grown cells. For a cell concentration of 4 mg per ml, maximal inhibition was obtained at 120 μg per ml. Greater concentrations did not increase the inhibitory effect. Cerium inhibited 14CO2 evolution and 14C uptake from uniformly labeled glucose. Marked changes in the distribution of 14C incorporated into different chemical fractions of the cell were noted. The most striking changes occurred in the alcohol- and alcohol ether-soluble fractions, in which the 14C activity was increased 5- to 20-fold in the presence of cerium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLIFTON C. E., SOBEK J. M. Endogenous respiration of Bacillus cereus. J Bacteriol. 1961 Aug;82:252–256. doi: 10.1128/jb.82.2.252-256.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Good N. E., Winget G. D., Winter W., Connolly T. N., Izawa S., Singh R. M. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467–477. doi: 10.1021/bi00866a011. [DOI] [PubMed] [Google Scholar]
- JOHNSON G. T., KYKER G. C. Fission-product and cerium uptake by bacteria, yeasts, and molds. J Bacteriol. 1961 May;81:733–740. doi: 10.1128/jb.81.5.733-740.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson G. T., Kyker G. C. The mechanism of cerium uptake by Saccharomyces cerevisiae. Mycologia. 1966 Jan-Feb;58(1):91–99. [PubMed] [Google Scholar]
- WURM M. The effect of lanthanum on growth and metabolism of Streptococcus faecalis R. J Biol Chem. 1951 Oct;192(2):707–714. [PubMed] [Google Scholar]