Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Jan;95(1):157–161. doi: 10.1128/jb.95.1.157-161.1968

Activation of Fatty Acid Synthesis in Cell-free Extracts of Saccharomyces cerevisiae

Roberta K Rasmussen 1, Harold P Klein 1
PMCID: PMC251986  PMID: 5636813

Abstract

Fatty acid synthesis from acetate in extracts of Saccharomyces cerevisiae strain LK2G12 was shown to be stimulated by α-glycerophosphate and citrate, and by a number of compounds related to them. Magnesium was shown to stimulate fatty acid synthesis from acetyl-coenzyme A but not from malonyl-coenzyme A, thus indicating the site of stimulation of fatty acid synthesis to be the acetyl-coenzyme A step.

Full text

PDF
157

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAHAM S., CHAIKOFF I. L. Glycolytic pathways and lipogenesis in mammary glands of lactating and nonlactating normal rats. J Biol Chem. 1959 Sep;234:2246–2253. [PubMed] [Google Scholar]
  2. ABRAHAM S., LORCH E., CHAIKOFF I. L. Localization of the stimulating effect of isocitrate on fatty acid synthesis by rat liver homogenate fractions. Biochem Biophys Res Commun. 1962 Apr 20;7:190–193. doi: 10.1016/0006-291x(62)90172-9. [DOI] [PubMed] [Google Scholar]
  3. ABRAHAM S., MATTHES K. J., CHAIKOFF I. L. Factors involved in synthesis of fatty acids from acetate by a soluble fraction obtained from lactating rat mammary gland. Biochim Biophys Acta. 1961 May 13;49:268–285. doi: 10.1016/0006-3002(61)90127-5. [DOI] [PubMed] [Google Scholar]
  4. DILS R., POPJAK G. Biosynthesis of fatty acids in cell-free preparations. 5. Synthesis of fatty acids from acetate in extracts of lactating-rat mammary gland. Biochem J. 1962 Apr;83:41–51. doi: 10.1042/bj0830041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EVANS H. J., CLARK R. B., RUSSELL S. A. CATION REQUIREMENTS FOR THE ACETIC THIOKINASE FROM YEAST. Biochim Biophys Acta. 1964 Dec 23;92:582–594. doi: 10.1016/0926-6569(64)90018-5. [DOI] [PubMed] [Google Scholar]
  6. GANGULY J. Studies on the mechanism of fatty acid synthesis. VII. Biosynthesis of fatty acids from malonyl CoA. Biochim Biophys Acta. 1960 May 6;40:110–118. doi: 10.1016/0006-3002(60)91320-2. [DOI] [PubMed] [Google Scholar]
  7. Gregolin C., Ryder E., Kleinschmidt A. K., Warner R. C., Lane M. D. Molecular characteristics of liver acetyl CoA carboxylase. Proc Natl Acad Sci U S A. 1966 Jul;56(1):148–155. doi: 10.1073/pnas.56.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gregolin C., Ryder E., Warner R. C., Kleinschmidt A. K., Lane M. D. Liver acetyl coa carboxylase: the dissociation-reassociation process and its relation to catalytic activity. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1751–1758. doi: 10.1073/pnas.56.6.1751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HOWARD C. F., LOWENSTEIN J. M. THE EFFECT OF ALPH-GLYCEROPHOSPHATE ON THE MICROSOMAL STIMULATION OF FATTY ACID SYNTHESIS. Biochim Biophys Acta. 1964 Apr 20;84:226–228. doi: 10.1016/0926-6542(64)90089-7. [DOI] [PubMed] [Google Scholar]
  10. KLEIN H. P. STIMULATION OF LIPID SYNTHESIS BY YEAST RIBOSOMAL PREPARATIONS. Biochim Biophys Acta. 1963 Oct 22;70:606–608. doi: 10.1016/0006-3002(63)90803-5. [DOI] [PubMed] [Google Scholar]
  11. Klein H. P. Synthesis of fatty acids by yeast particles. J Bacteriol. 1966 Jul;92(1):130–135. doi: 10.1128/jb.92.1.130-135.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MARTIN D. B., VAGELOS P. R. The mechanism of tricarboxylic acid cycle regulation of fatty acid synthesis. J Biol Chem. 1962 Jun;237:1787–1792. [PubMed] [Google Scholar]
  14. MATSUHASHI M., MATSUHASHI S., LYNEN F. ZUR BIOSYNTHESE DER FETTSAEUREN. V. DIE ACETYL-COA CARBOXYLASE AUS RATTENLEBER UND IHRE AKTIVIERUNG DURCH CITRONENSAEURE. Biochem Z. 1964 Aug 11;340:263–289. [PubMed] [Google Scholar]
  15. Numa S., Ringelmann E., Riedel B. Further evidence for polymeric structure of liver acetyl CoA carboxylase. Biochem Biophys Res Commun. 1966 Sep 8;24(5):750–757. doi: 10.1016/0006-291x(66)90389-5. [DOI] [PubMed] [Google Scholar]
  16. Rasmussen R. K., Klein H. P. Regulation of acetyl-CoA carboxylase of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1967 Aug 7;28(3):415–419. doi: 10.1016/0006-291x(67)90327-0. [DOI] [PubMed] [Google Scholar]
  17. VAGELOS P. R., ALBERTS A. W., MARTIN D. B. Studies on the mechnism of activation of acetyl coenzyme A carboxylase by citrate. J Biol Chem. 1963 Feb;238:533–540. [PubMed] [Google Scholar]
  18. WHITE D., KLEIN H. P. FACTORS AFFECTING FATTY ACID SYNTHESIS IN CELL-FREE PREPARATIONS FROM SACCHAROMYCES CEREVISIAE. Biochem Biophys Res Commun. 1965 Jun 18;20:78–84. doi: 10.1016/0006-291x(65)90953-8. [DOI] [PubMed] [Google Scholar]
  19. White D., Klein H. P. Effects of alpha-glycerophosphate and of palmityl-coenzyme A on lipid synthesis in yeast extracts. J Bacteriol. 1966 Mar;91(3):1218–1223. doi: 10.1128/jb.91.3.1218-1223.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES