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. 1968 Feb;95(2):304–309. doi: 10.1128/jb.95.2.304-309.1968

Initiation of Deoxyribonucleic Acid Synthesis After Thymine Starvation of Bacillus subtilis

Neville R Kallenbach 1, Rong-Ine Ma 1
PMCID: PMC252018  PMID: 4966542

Abstract

Evidence for premature initiation of deoxyribonucleic acid (DNA) replication after thymine starvation of Bacillus subtilis W23T− is presented, based on (i) increase in the number of ade+ relative to met+ transformants yielded by the DNA isolated from cultures after starvation (the ade− marker being near the origin of replication, whereas met− is close to the terminus), and (ii) increase in both the initial rate and final level of tritiated thymine incorporation in the presence of chloramphenicol after release from starvation. The marker ratio data agree quantitatively with the hypothesis that the initiation is induced only on one arm of each chromosome which was replicating prior to starvation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARNER H. D., COHEN S. S. Synchronization of division of a thymineless mutant of Escherichia coli. J Bacteriol. 1956 Jul;72(1):115–123. doi: 10.1128/jb.72.1.115-123.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BERNS K. I., THOMAS C. A., Jr ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:476–490. doi: 10.1016/s0022-2836(65)80004-3. [DOI] [PubMed] [Google Scholar]
  4. BONHOEFFER F., GIERER A. ON THE GROWTH MECHANISM OF THE BACTERIAL CHROMOSOME. J Mol Biol. 1963 Nov;7:534–540. doi: 10.1016/s0022-2836(63)80100-x. [DOI] [PubMed] [Google Scholar]
  5. CROTHERS D. M. THE KINETICS OF DNA DENATURATION. J Mol Biol. 1964 Sep;9:712–733. doi: 10.1016/s0022-2836(64)80177-7. [DOI] [PubMed] [Google Scholar]
  6. Cohen S. S., Barner H. D. STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1954 Oct;40(10):885–893. doi: 10.1073/pnas.40.10.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cohen S. S., Flaks J. G., Barner H. D., Loeb M. R., Lichtenstein J. THE MODE OF ACTION OF 5-FLUOROURACIL AND ITS DERIVATIVES. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1004–1012. doi: 10.1073/pnas.44.10.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FARMER J. L., ROTHMAN F. TRANSFORMABLE THYMINE-REQUIRING MUTANT OF BACILLUS SUBTILS. J Bacteriol. 1965 Jan;89:262–263. doi: 10.1128/jb.89.1.262-263.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GALLANT J. A. Sublethal thymineless damage in Escherichia coliB3. Biochim Biophys Acta. 1962 Aug 20;61:302–304. [PubMed] [Google Scholar]
  10. Gallant J., Spottswood T. The recombinogenic effect of thymidylate starvation in Escherichia coli merodiploids. Genetics. 1965 Jul;52(1):107–118. doi: 10.1093/genetics/52.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HANAWALT P. C. Involvement of synthesis of RNA in thymineless death. Nature. 1963 Apr 20;198:286–286. doi: 10.1038/198286a0. [DOI] [PubMed] [Google Scholar]
  12. KANAZIR D. The apparent mutagenicity of thymine deficiency. Biochim Biophys Acta. 1958 Oct;30(1):20–23. doi: 10.1016/0006-3002(58)90235-x. [DOI] [PubMed] [Google Scholar]
  13. KECK K. An ultramicro technique for the determination of deoxypentose nucleic acid. Arch Biochem Biophys. 1956 Aug;63(2):446–451. doi: 10.1016/0003-9861(56)90059-5. [DOI] [PubMed] [Google Scholar]
  14. Lark K. G., Bird R. Premature chromosome replication induced by thymine starvation: restriction of replication to one of the two partially completed replicas. J Mol Biol. 1965 Sep;13(2):607–610. doi: 10.1016/s0022-2836(65)80125-5. [DOI] [PubMed] [Google Scholar]
  15. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Luzzati D. Effect of thymine starvation on messenger ribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1966 Nov;92(5):1435–1446. doi: 10.1128/jb.92.5.1435-1446.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  18. O'Sullivan A., Sueoka N. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment. J Mol Biol. 1967 Jul 28;27(2):349–368. doi: 10.1016/0022-2836(67)90025-3. [DOI] [PubMed] [Google Scholar]
  19. PRITCHARD R. H., LARK K. G. INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI. J Mol Biol. 1964 Aug;9:288–307. doi: 10.1016/s0022-2836(64)80208-4. [DOI] [PubMed] [Google Scholar]
  20. Rolfe R. On the mechanism of thymineless death in Bacillus subtilis. Proc Natl Acad Sci U S A. 1967 Jan;57(1):114–121. doi: 10.1073/pnas.57.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SICARD N., ANAGNOSTOPOULOS C. ACTIVIT'E TRANSFORMANTE DE L'ACIDE D'ESOXYRIBONUCL'EIQUE DE BACILLUS SUBTILIS LORS DE LA CARENCE EN THYMINE. C R Hebd Seances Acad Sci. 1964 Nov 30;259:4173–4176. [PubMed] [Google Scholar]
  22. SICARD N., DEVORET R. [Effects of thymine dificiency on the K 12 T lysogenic and 15 T colicinogenic strains of Escherichia coli]. C R Hebd Seances Acad Sci. 1962 Sep 10;255:1417–1419. [PubMed] [Google Scholar]
  23. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. YOSHIKAWA H., SUEOKA N. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases. Proc Natl Acad Sci U S A. 1963 Apr;49:559–566. doi: 10.1073/pnas.49.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yoshikawa H. DNA synthesis during germination of Bacillus subtilis spores. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1476–1483. doi: 10.1073/pnas.53.6.1476. [DOI] [PMC free article] [PubMed] [Google Scholar]

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