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. 1968 Feb;95(2):355–359. doi: 10.1128/jb.95.2.355-359.1968

Microcycle Sporogenesis of Bacillus cereus in a Chemically Defined Medium

Ian MacKechnie 1, Richard S Hanson 1
PMCID: PMC252026  PMID: 4967879

Abstract

A chemically defined medium which allowed germination, outgrowth, and subsequent resporulation of Bacillus cereus T spores, without intervening cell division (microcycle sporogenesis), is described. No medium replacement was required. The second-stage spores were heat-stable and had similar germination characteristics and dipicolinic acid content to primary spores. Deoxyribonucleic acid (DNA) replication began soon after germination and there was a doubling in the DNA content of the cells within 2 hr.

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Selected References

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