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. 1968 Feb;95(2):406–417. doi: 10.1128/jb.95.2.406-417.1968

Antibody Response and Protection Induced by Immunization with Smooth and Rough Strains in Experimental Salmonellosis1

Kathryn Kenny a,2, Mendel Herzberg a,3
PMCID: PMC252033  PMID: 4867738

Abstract

The antibody response of mice to a smooth strain of Salmonella typhimurium was shown previously to be extremely rapid and potent. As measured by the complement-mediated bactericidal reaction, it was also found to be highly specific as well as reproducible. Experiments which studied the effects of antigen type (live or heat-killed), antigen dose, and the route of immunization indicated that the most rapid and highest antibody response was achieved with live, smooth organisms injected by the intraperitoneal route. Living vaccines of rough strains of either S. typhimurium or S. enteritidis induced antibodies directed against the corresponding smooth organisms. The response to the rough strains was apparently due to antibody production rather than to the simple release of preformed natural antibody. The duration of protection conferred by the rough strain vaccines was closely correlated with the endotoxic content of the immunizing strain. Smooth heat-killed vaccines and a rough live vaccine protected against homologous but not heterologous challenge. In contrast, immunization with a smooth live vaccine protected mice against both homologous and heterologous challenge infections. Protection was not due to a local effect in the peritoneal cavity, since mice were also protected against subcutaneous challenge. The secondary antibody response, induced in immunized animals by the virulent challenge infection, was demonstrated to be rapid and potent, and hence a factor to be considered in protection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AKIYAMA T., MAEDA K., USHIBA D. [Studies on immunity in experimental typhoid. Challenge of mice passively immunized with antiserum through various routes (mechanism of immunization with killed vaccines)]. Nihon Saikingaku Zasshi. 1962 Oct;17:829–834. [PubMed] [Google Scholar]
  2. BECKMANN I., SUBBAIAH T. V., STOCKER B. A. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. II. SEROLOGICAL AND CHEMICAL INVESTIGATIONS. Nature. 1964 Mar 28;201:1299–1301. doi: 10.1038/2011299a0. [DOI] [PubMed] [Google Scholar]
  3. Blanden R. V., Mackaness G. B., Collins F. M. Mechanisms of acquired resistance in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):585–600. doi: 10.1084/jem.124.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins F. M., Mackaness G. B., Blanden R. V. Infection-immunity in experimental salmonellosis. J Exp Med. 1966 Oct 1;124(4):601–619. doi: 10.1084/jem.124.4.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gemski P., Jr, Stocker B. A. Transduction by bacteriophage P22 in nonsmooth mutants of Salmonella typhimurium. J Bacteriol. 1967 May;93(5):1588–1597. doi: 10.1128/jb.93.5.1588-1597.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HERZBERG M., JAWAD M. J., PRATT D. CORRELATION OF SUCCINATE METABOLISM AND VIRULENCE IN SALMONELLA TYPHIMURIUM. J Bacteriol. 1965 Jan;89:185–192. doi: 10.1128/jb.89.1.185-192.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOBSON D. Resistance to reinfection in experimental mouse typhoid. J Hyg (Lond) 1957 Sep;55(3):334–343. doi: 10.1017/s0022172400037244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kenny K., Herzberg M. Early antibody response in mice to either infection or immunization with Salmonella typhimurium. J Bacteriol. 1967 Mar;93(3):773–778. doi: 10.1128/jb.93.3.773-778.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LARSON C. L., BELL J. F., OWEN C. R. The development of resistance in mice immunized with soluble antigen derived from Bacterium tularense. J Immunol. 1954 Oct;73(4):221–225. [PubMed] [Google Scholar]
  10. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MITSUHASHI S., KAWAKAMI M., YAMAGUCHI Y., NAGAI M. Studies on the experimental typhoid. 1. A comparative study of living and killed vaccines against the infection of mice with S. enteritidis. Jpn J Exp Med. 1958 Aug;28(4):249–258. [PubMed] [Google Scholar]
  12. Michael J. G. The release of specific bactericidal antibodies by endotoxin. J Exp Med. 1966 Feb 1;123(2):205–212. doi: 10.1084/jem.123.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NAKANO M. Mutants of Salmonella with unusually low toxicity for mice. Nature. 1962 Dec 15;196:1118–1119. doi: 10.1038/1961118a0. [DOI] [PubMed] [Google Scholar]
  14. USHIBA D., SAITO K., AKIYAMA T., NAKANO M., SUGIYAMA T., SHIRONO S. Studies on experimental typhoid: bacterial multiplication and host cell response after infection with Salmonella enteritidis in mice immunized with live and killed vaccines. Jpn J Microbiol. 1959 Apr;3:231–242. doi: 10.1111/j.1348-0421.1959.tb00119.x. [DOI] [PubMed] [Google Scholar]
  15. Ushiba D., Nakae T., Akiyama T., Kishimoto Y. Characterization of "clearance" factor and "cell-bound" antibody in experimental typhoid. J Bacteriol. 1966 May;91(5):1705–1712. doi: 10.1128/jb.91.5.1705-1712.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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