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. 1968 Feb;95(2):618–624. doi: 10.1128/jb.95.2.618-624.1968

Antigenic Relationship of the Gram-negative Organism Causing Canine Abortion to Smooth and Rough Brucellae

Ramon Diaz 1, Lois M Jones 1, J B Wilson 1
PMCID: PMC252060  PMID: 4295848

Abstract

The use of whole-cell antigens in agglutination and agglutinin-absorption tests showed that the organism causing abortion in dogs is similar to rough Brucella abortus, B. melitensis, and B. ovis, but different from smooth Brucella cultures. Water-soluble antigens obtained by ultrasonic treatment and examined by immuno-electrophoresis and gel diffusion show extensive cross-reactions within the genus Brucella, but little or no cross-reaction with similar antigens from other gram-negative genera in the family Brucellaceae. The dog organism showed near identity with rough and smooth Brucella cultures on the basis of immuno-gel diffusion tests with water-soluble antigens, but it lacked the lipopolysaccharide-endotoxin associated with the agglutinogen of smooth brucellae. These findings support the proposal of Carmichael and Bruner for the designation of a new species, “Brucella canis.”

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. J., Wilson J. B. Chemical composition and biological properties of the endotoxin of Brucella abortus. J Bacteriol. 1965 Oct;90(4):895–902. doi: 10.1128/jb.90.4.895-902.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Currie J. A., Marshall J. D., Jr, Crozier D. The detection of Pasteurella pseudotuberculosis antibodies by the microhemagglutination test. J Infect Dis. 1966 Apr;116(2):117–122. doi: 10.1093/infdis/116.2.117. [DOI] [PubMed] [Google Scholar]
  3. Diaz R., Jones L. M., Leong D., Wilson J. B. Differences between Brucella antigens involved in indirect hemagglutination tests with normal and tanned red blood cells. J Bacteriol. 1967 Sep;94(3):499–505. doi: 10.1128/jb.94.3.499-505.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diaz R., Jones L. M., Wilson J. B. Antigenic relationship of Brucella ovis and Brucella melitensis. J Bacteriol. 1967 Apr;93(4):1262–1268. doi: 10.1128/jb.93.4.1262-1268.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones L. M., Zanardi M., Leong D., Wilson J. B. Taxonomic position in the genus Brucella of the causative agent of canine abortion. J Bacteriol. 1968 Feb;95(2):625–630. doi: 10.1128/jb.95.2.625-630.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. OLITZKI A. L., GODINGER D. INTER-FAMILIAR ANTIGENIC RELATIONSHIPS BETWEEN ENTEROBACTERIACEAE, BRUCELLACEAE AND PSEUDOMONADACEAE REVEALED BY THE AGAR GEL PRECIPITATION TECHNIQUE. Boll Ist Sieroter Milan. 1963 May-Jun;42:213–232. [PubMed] [Google Scholar]
  7. OLITZKI A. L. The antigenic structure of the Brucellae. Boll Ist Sieroter Milan. 1960 Mar-Apr;39:97–104. [PubMed] [Google Scholar]
  8. Prince G. H., Smith J. E. Antigenic studies on Pasteurella multocida using immunodiffusion techniques. II. Relationships with other gram-negative species. J Comp Pathol. 1966 Jul;76(3):315–320. doi: 10.1016/0021-9975(66)90012-0. [DOI] [PubMed] [Google Scholar]
  9. RIBI E., MILNER K. C., PERRINE T. D. Endotoxic and antigenic fractions from the cell wall of Salmonella enteritidis; methods for separation and some biologic activities. J Immunol. 1959 Jan;82(1):75–84. [PubMed] [Google Scholar]

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