Abstract
The distribution of uninucleate and multinucleate cells of Bacillus subtilis, fractionated by zonal centrifugation, shows that the uninucleate cells are most likely to be competent. Only the competent fraction of the population incorporates deoxyribonucleic acid.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cahn F. H., Fox M. S. Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients. J Bacteriol. 1968 Mar;95(3):867–875. doi: 10.1128/jb.95.3.867-875.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GANESAN A. T., LEDERBERG J. PHYSICAL AND BIOLOGICAL STUDIES ON TRANSFORMING DNA. J Mol Biol. 1964 Sep;9:683–695. doi: 10.1016/s0022-2836(64)80175-3. [DOI] [PubMed] [Google Scholar]
- KELLY M. S., PRITCHARD R. H. UNSTABLE LINKAGE BETWEEN GENETIC MARKERS IN TRANSFORMATION. J Bacteriol. 1965 May;89:1314–1321. doi: 10.1128/jb.89.5.1314-1321.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kammen H. O., Beloff R. H., Canellakis E. S. Transformation in Bacillus subtilis. I. Role of amino acids in stabilization of transformants. Biochim Biophys Acta. 1966 Jul 20;123(1):39–55. [PubMed] [Google Scholar]
- Kammen H. O., Wojnar R. J., Canellakis E. S. Transformation in Bacillus subtilis. II. The development and maintenance of the competent state. Biochim Biophys Acta. 1966 Jul 20;123(1):56–65. [PubMed] [Google Scholar]
- LERMAN L. S., TOLMACH L. J. Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus. Biochim Biophys Acta. 1957 Oct;26(1):68–82. doi: 10.1016/0006-3002(57)90055-0. [DOI] [PubMed] [Google Scholar]
- MARMUR J., GROSSMAN L. Ultraviolet light induced linking of deoxyribonucleic acid strands and its reversal by photoreactivating enzyme. Proc Natl Acad Sci U S A. 1961 Jun 15;47:778–787. doi: 10.1073/pnas.47.6.778. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NESTER E. W., STOCKER B. A. BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1963 Oct;86:785–796. doi: 10.1128/jb.86.4.785-796.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nester E W, Schafer M, Lederberg J. Gene Linkage in DNA Transfer: A Cluster of Genes Concerned with Aromatic Biosynthesis in Bacillus Subtilis. Genetics. 1963 Apr;48(4):529–551. doi: 10.1093/genetics/48.4.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
- SMITH A. G. Electron and light microscopic studies of bacterial nuclei. II. An improved standing technique for the nuclear chromatin of bacterial cells. J Bacteriol. 1950 Apr;59(4):575–587. doi: 10.1128/jb.59.4.575-587.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YOUNG F. E., SPIZIZEN J. Physiological and genetic factors affecting transformation of Bacillus subtilis. J Bacteriol. 1961 May;81:823–829. doi: 10.1128/jb.81.5.823-829.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young F. E. Competence in Bacillus subtilis transformation system. Nature. 1967 Feb 25;213(5078):773–775. doi: 10.1038/213773a0. [DOI] [PubMed] [Google Scholar]
- Young F. E., Jackson A. P. Extent and significance of contamination of DNA by teichoic acid in Bacillus subtilis. Biochem Biophys Res Commun. 1966 May 25;23(4):490–495. doi: 10.1016/0006-291x(66)90755-8. [DOI] [PubMed] [Google Scholar]