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. 1968 Mar;95(3):947–951. doi: 10.1128/jb.95.3.947-951.1968

Microbial Degradation of Corrinoids VI. Reduction of Hydroxocobalamin by Cell-free Particles from Pseudomonas rubescens

J B Hufham a,1, R C Burgus a,2, Sandra Y S Ing a, J J Pfiffner a
PMCID: PMC252114  PMID: 4384487

Abstract

Cell-free particles from Pseudomonas rubescens have been shown to reduce hydroxocobalamin to vitamin B12r. The particles are unable to reduce the B12r to B12s. The reduction of hydroxocobalamin is dependent upon reduced nicotinamide adenine dinucleotide and is stimulated by flavin adenine dinucleotide. Cobinamide and diaquocobinamide were reduced at 25 and 10%, respectively, of the rate of hydroxocobalamin. Cyanocobalamin, coenzyme B12, pseudovitamin B12, and diaquopseudocobalamin were not reduced. Reduced nicotinamide adenine dinucleotide phosphate and flavin mononucleotide were not active. Diaphorase and xanthine oxidase activity were not present in the particulate fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGUS R. C., HUFHAM J. B., SCOTT W. M., PFIFFNER J. J. MICROBIAL DEGRADATION OF CORRINOIDS. 3. PIGMENTS DERIVED FROM VITAMIN B12 BY PSEUDOMONAS RUBESCENS. J Bacteriol. 1964 Oct;88:1139–1144. doi: 10.1128/jb.88.4.1139-1144.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HUFHAM J. B., BURGUS R. C., SCOTT W. M., PFIFFNER J. J. REDUCTION OF VITAMIN B12 BY PSEUDOMONAS RUBESCENS. J Bacteriol. 1964 Aug;88:538–540. doi: 10.1128/jb.88.2.538-540.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. SCOTT W. M., BURGUS R. C., HUFHAM J. B., PFIFFNER J. J. MICROBIAL DEGRADATION OF CORRINOIDS. I. VITAMIN B12. J Bacteriol. 1964 Sep;88:581–585. doi: 10.1128/jb.88.3.581-585.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. TAKEYAMA S., BUCHANAN J. M. Enzymatic synthesis of the methyl group of methionine. III. Spectral and electrophoretic studies of the prosthetic group of the B12 enzyme. J Biochem. 1961 Jun;49:578–588. doi: 10.1093/oxfordjournals.jbchem.a127347. [DOI] [PubMed] [Google Scholar]
  5. Vitols E., Walker G. A., Huennekens F. M. Enzymatic conversion of vitamin B-12s to a cobamide coenzyme, alpha-(5,6-dimethylbenzimidazolyl)deoxyadenosylcobamide (adenosyl-B-12). J Biol Chem. 1966 Apr 10;241(7):1455–1461. [PubMed] [Google Scholar]
  6. WEISSBACH H., REDFIELD B. G., DICKERMAN H., BROT N. STUDIES ON METHIONINE BIOSYNTHESIS. EFFECT OF ALKYLCOBAMIDE DERIVATIVES ON THE FORMATION OF HOLOENZYME. J Biol Chem. 1965 Feb;240:856–862. [PubMed] [Google Scholar]

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