Abstract
Studies of luminescence in growing cultures of Photobacterium fischeri revealed the characteristic kinetics of light emission, including a minimal phase of bacterial light output. A dialyzable substance present in the nutrient broth medium caused this transient inhibition in light production, although this substance did not affect culture growth. Experiments were carried out to determine the mechanism of action and the chemical properties of the inhibitor. The results suggest that the inhibitor may be binding directly to the luciferase molecules.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HASTINGS J. W., RILEY W. H., MASSA J. THE PURIFICATION PROPERTIES, AND CHEMILUMINESCENT QUANTUM YIELD OF BACTERIAL LUCIFERASE. J Biol Chem. 1965 Mar;240:1473–1481. [PubMed] [Google Scholar]
- Kempner E. S. Trace metal analysis of nutrient broth. Appl Microbiol. 1967 Nov;15(6):1525–1526. doi: 10.1128/am.15.6.1525-1526.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]