Abstract
A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.
Full text
PDF![991](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/252122/e57d97d71ebc/jbacter00401-0289.png)
![992](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/252122/1a8ffeb14af1/jbacter00401-0290.png)
![993](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/252122/b3985db13c50/jbacter00401-0291.png)
![994](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/252122/6dd38594968c/jbacter00401-0292.png)
![995](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/252122/05476ee517ed/jbacter00401-0293.png)
![996](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/252122/3de6270f6a8e/jbacter00401-0294.png)
![997](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a59/252122/311589f6a4e8/jbacter00401-0295.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown K. D., Doy C. H. Control of three isoenzymic 7-phospho-2-oxo-3-deoxy-Darabino-heptonate-D-erythrose-4-phosphate lyases of Escherichia coli W and derived mutants by repressive and "inductive" effects of the aromatic amino acids. Biochim Biophys Acta. 1966 Apr 12;118(1):157–172. doi: 10.1016/s0926-6593(66)80153-4. [DOI] [PubMed] [Google Scholar]
- EDLIN G. AMINO ACID REGULATION OF BACTERIOPHAGE RNA SYNTHESIS. J Mol Biol. 1965 Jun;12:356–362. doi: 10.1016/s0022-2836(65)80259-5. [DOI] [PubMed] [Google Scholar]
- EIDLIC L., NEIDHARDT F. C. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES. J Bacteriol. 1965 Mar;89:706–711. doi: 10.1128/jb.89.3.706-711.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FREUNDLICH M., BURNS R. O., UMBARGER H. E. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804–1808. doi: 10.1073/pnas.48.10.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KORN D., WEISSBACH A. Thymineless induction in Escherichia coli K12 (lambda). Biochim Biophys Acta. 1962 Nov 26;61:775–790. doi: 10.1016/0926-6550(62)90060-9. [DOI] [PubMed] [Google Scholar]
- KURLAND C. G., MAALOE O. Regulation of ribosomal and transfer RNA synthesis. J Mol Biol. 1962 Mar;4:193–210. doi: 10.1016/s0022-2836(62)80051-5. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris D. W., DeMoss J. A. Polysome transitions and the regulation of ribonucleic acid synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1966 Jul;56(1):262–268. doi: 10.1073/pnas.56.1.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NEIDHARDT F. C. Properties of a bacterial mutant lacking amino acid control of RNA synthesis. Biochim Biophys Acta. 1963 Mar 26;68:365–379. doi: 10.1016/0006-3002(63)90158-6. [DOI] [PubMed] [Google Scholar]
- Neidhardt F. C. Roles of amino acid activating enzymes in cellular physiology. Bacteriol Rev. 1966 Dec;30(4):701–719. doi: 10.1128/br.30.4.701-719.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OKAGAKI H., TSUBOTA Y., SIBATANI A. Unbalanced growth and bacterial death in thymine-deficient and ultraviolet irradiated Escherichia coli. J Bacteriol. 1960 Dec;80:762–771. doi: 10.1128/jb.80.6.762-771.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rolfe R. On the mechanism of thymineless death in Bacillus subtilis. Proc Natl Acad Sci U S A. 1967 Jan;57(1):114–121. doi: 10.1073/pnas.57.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roth J. R., Ames B. N. Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase. J Mol Biol. 1966 Dec 28;22(2):325–333. doi: 10.1016/0022-2836(66)90135-5. [DOI] [PubMed] [Google Scholar]
- SOMERVILLE R. L., YANOFSKY C. STUDIES ON THE REGULATION OF TRYPTOPHAN BIOSYNTHESIS IN ESCHERICHIA COLI. J Mol Biol. 1965 Apr;11:747–759. doi: 10.1016/s0022-2836(65)80032-8. [DOI] [PubMed] [Google Scholar]
- Wachsman J. T., Hogg L. Use of thymineless death to enrich for doubly auxotrophic mutants of Bacillus megaterium. J Bacteriol. 1964 May;87(5):1118–1122. doi: 10.1128/jb.87.5.1118-1122.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]