Abstract
From Escherichia coli B, mutants were prepared that lacked the enzymes adenosine deaminase, cytidine deaminase, and purine nucleoside phosphorylase. In each case, the mutant lacked enzyme activity for both ribonucleoside and deoxyribonucleoside. Mutants lacking purine nucleoside phosphorylase lost the capacity to cleave the nucleosides of adenine, guanine, and hypoxanthine.
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Selected References
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- Alikhanian S. I., Iljina T. S., Kaliaeva E. S., Kameneva S. V., Sukhodolec V. V. A genetical study of thymineless mutants of E. coli K12. Genet Res. 1966 Aug;8(1):83–100. doi: 10.1017/s0016672300009939. [DOI] [PubMed] [Google Scholar]
- Boyer H. Conjugation in Escherichia coli. J Bacteriol. 1966 May;91(5):1767–1772. doi: 10.1128/jb.91.5.1767-1772.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN D. A variant of phage P2 originating in Escherichia coli, strain B. Virology. 1959 Jan;7(1):112–126. doi: 10.1016/0042-6822(59)90180-1. [DOI] [PubMed] [Google Scholar]
- COHEN S. S., BARNER H. D. The conversion of 5-methyldeoxycytidine to thymidine in vitro and in vivo. J Biol Chem. 1957 Jun;226(2):631–642. [PubMed] [Google Scholar]
- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FARMER J. L., ROTHMAN F. TRANSFORMABLE THYMINE-REQUIRING MUTANT OF BACILLUS SUBTILS. J Bacteriol. 1965 Jan;89:262–263. doi: 10.1128/jb.89.1.262-263.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FINK K., CLINE R. E., HENDERSON R. B., FINK R. M. Metabolism of thymine (methyl-C14 or -2-C14) by rat liver in vitro. J Biol Chem. 1956 Jul;221(1):425–433. [PubMed] [Google Scholar]
- GORINI L., KATAJA E. PHENOTYPIC REPAIR BY STREPTOMYCIN OF DEFECTIVE GENOTYPES IN E. COLI. Proc Natl Acad Sci U S A. 1964 Mar;51:487–493. doi: 10.1073/pnas.51.3.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GORINI L., KAUFMAN H. Selecting bacterial mutants by the penicillin method. Science. 1960 Feb 26;131(3400):604–605. doi: 10.1126/science.131.3400.604. [DOI] [PubMed] [Google Scholar]
- KOCH A. L., LAMONT W. A. The metabolism of methylpurines by Escherichia coli. II. Enzymatic studies. J Biol Chem. 1956 Mar;219(1):189–201. [PubMed] [Google Scholar]
- KOCH A. L., VALLEE G. The properties of adenosine deaminase and adenosine nucleoside phosphorylase in extracts of Escherichia coli. J Biol Chem. 1959 May;234(5):1213–1218. [PubMed] [Google Scholar]
- KORNBERG A., LIEBERMAN I., SIMMS E. S. Enzymatic synthesis of purine nucleotides. J Biol Chem. 1955 Jul;215(1):417–427. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Love S. H., Remy C. N. Metabolism of methylated purines in Escherichia coli: derepression of purine biosynthesis. J Bacteriol. 1966 Mar;91(3):1037–1049. doi: 10.1128/jb.91.3.1037-1049.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANSON L. A., LAMPEN J. O. The metabolism of desoxyribose nucleosides in Escherichia coli. J Biol Chem. 1951 Dec;193(2):539–547. [PubMed] [Google Scholar]
- MARKHAM R., SMITH J. D. Chromatographic studies of nucleic acids; a technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substances. Biochem J. 1949;45(3):294–298. doi: 10.1042/bj0450294. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOVICK R. P., MAAS W. K. Control by endogenously synthesized arginine of the formation of ornithine transcarbamylase in Escherichia coli. J Bacteriol. 1961 Feb;81:236–240. doi: 10.1128/jb.81.2.236-240.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OKADA T., HOMMA J., SONOHARA H. Improved method for obtaining thymineless mutants of Escherichia coli and Salmonella typhimurium. J Bacteriol. 1962 Sep;84:602–603. doi: 10.1128/jb.84.3.602-603.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OTT J. L., WERKMAN C. H. Coupled nucleoside phosphorylase reactions in Escherichia coli. Arch Biochem Biophys. 1957 Jul;69:264–276. doi: 10.1016/0003-9861(57)90491-5. [DOI] [PubMed] [Google Scholar]
- RAZZELL W. E., CASSHYAP P. SUBSTRATE SPECIFICITY AND INDUCTION OF THYMIDINE PHOSPHORYLASE IN ESCHERICHIA COLI. J Biol Chem. 1964 Jun;239:1789–1793. [PubMed] [Google Scholar]
- STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sasaki I., Bertani G. Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J Gen Microbiol. 1965 Sep;40(3):365–376. doi: 10.1099/00221287-40-3-365. [DOI] [PubMed] [Google Scholar]
- WANG T. P., SABLE H. Z., LAMPEN J. O. Enzymatic deamination of cytosine nucleosides. J Biol Chem. 1950 May;184(1):17–28. [PubMed] [Google Scholar]