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. 1968 Mar;95(3):1103–1110. doi: 10.1128/jb.95.3.1103-1110.1968

Osmotic Remedial Response in a Galactose-negative Mutant of Saccharomyces cerevisiae

John Bassel 1, H C Douglas 2
PMCID: PMC252137  PMID: 5643050

Abstract

We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective in inducing the osmotic remedial response. The galactose pathway enzymes were separable by ultracentrifugation, indicating that a stable interenzyme complex was not formed. The results were consistent with the hypothesis that the corrective effect occurs at the tertiary or quaternary level of organization in an environmentally sensitive protein. The possibility that the osmotic remedial response represents an effect of translation is discussed.

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Selected References

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  1. BROWN A. D. ASPECTS OF BACTERIAL RESPONSE TO THE IONIC ENVIRONMENT. Bacteriol Rev. 1964 Sep;28:296–329. doi: 10.1128/br.28.3.296-329.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CATCHESIDE D. G. INTERALLELIC COMPLEMENTATION. Brookhaven Symp Biol. 1964 Dec;17:1–14. [PubMed] [Google Scholar]
  3. DOUGLAS H. C. A mutation in saccharomyces that affects phosphoglucomutase activity and galactose utilization. Biochim Biophys Acta. 1961 Sep 2;52:209–211. doi: 10.1016/0006-3002(61)90924-6. [DOI] [PubMed] [Google Scholar]
  4. DOUGLAS H. C., HAWTHORNE D. C. ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES. Genetics. 1964 May;49:837–844. doi: 10.1093/genetics/49.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EDGAR R. S., DENHARDT G. H., EPSTEIN R. H. A COMPARATIVE GENETIC STUDY OF CONDITIONAL LETHAL MUTATIONS OF BACTERIOPHAGE T4D. Genetics. 1964 Apr;49:635–648. doi: 10.1093/genetics/49.4.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FINCHAM J. R., CODDINGTON A. Complementation at the am locus of Neurospora crassa: a reaction between different mutant forms of glutamate dehydrogenase. J Mol Biol. 1963 May;6:361–373. doi: 10.1016/s0022-2836(63)80049-2. [DOI] [PubMed] [Google Scholar]
  7. Fink G. R. A cluster of genes controlling three enzymes in histidine biosynthesis in Saccharomyces cerevisiae. Genetics. 1966 Mar;53(3):445–459. doi: 10.1093/genetics/53.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HAWTHORNE D. C., FRIIS J. OSMOTIC-REMEDIAL MUTANTS. A NEW CLASSIFICATION FOR NUTRITIONAL MUTANTS IN YEAST. Genetics. 1964 Nov;50:829–839. doi: 10.1093/genetics/50.5.829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HAWTHORNE D. C., MORTIMER R. K. Super-suppressors in yeast. Genetics. 1963 Apr;48:617–620. doi: 10.1093/genetics/48.4.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hawthorne D C, Mortimer R K. Chromosome Mapping in Saccharomyces: Centromere-Linked Genes. Genetics. 1960 Aug;45(8):1085–1110. doi: 10.1093/genetics/45.8.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KALCKAR H. M., BRAGANCA B., MUNCH-PETERSEN H. M. Uridyl transferases and the formation of uridine diphosphogalactose. Nature. 1953 Dec 5;172(4388):1038–1038. [PubMed] [Google Scholar]
  12. Kosterlitz H. W. The fermentation of galactose and galactose-1-phosphate. Biochem J. 1943 Sep;37(3):322–326. doi: 10.1042/bj0370322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LELOIR L. F. The enzymatic transformation of uridine diphosphate glucose into a galactose derivative. Arch Biochem Biophys. 1951 Sep;33(2):186–190. doi: 10.1016/0003-9861(51)90096-3. [DOI] [PubMed] [Google Scholar]
  14. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  15. Printz D. B., Gross S. R. An apparent relationship between mistranslation and an altered leucyl-tRNA synthetase in a conditional lethal mutant of Neurospora crassa. Genetics. 1967 Mar;55(3):451–467. doi: 10.1093/genetics/55.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SO A. G., DAVIE E. W. THE EFFECTS OF ORGANIC SOLVENTS ON PROTEIN BIOSYNTHESIS AND THEIR INFLUENCE ON THE AMINO ACID CODE. Biochemistry. 1964 Aug;3:1165–1169. doi: 10.1021/bi00896a027. [DOI] [PubMed] [Google Scholar]
  17. STRAUB F. B. FORMATION OF THE SECONDARY AND TERTIARY STRUCTURE OF ENZYMES. Adv Enzymol Relat Areas Mol Biol. 1964;26:89–114. doi: 10.1002/9780470122716.ch3. [DOI] [PubMed] [Google Scholar]
  18. WAGNER R. P., BERGQUIST A., BARBEE T., KIRITANI K. GENETIC BLOCKS IN THE ISOLEUCINE-VALINE PATHWAY OF NEUROSPORA CRASSA. Genetics. 1964 May;49:865–882. doi: 10.1093/genetics/49.5.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weinstein I. B., Friedman S. M., Ochoa M., Jr Fidelity during translation of the genetic code. Cold Spring Harb Symp Quant Biol. 1966;31:671–681. doi: 10.1101/sqb.1966.031.01.086. [DOI] [PubMed] [Google Scholar]
  20. Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]

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