Abstract
Five strains of Staphylococcus aureus were examined quantitatively for the production of nuclease under aerobic and anaerobic conditions. Hydrolysis of deoxyribonucleic acid and ribonucleic acid was detected by measuring the release of acidsoluble nucleotides spectrophotometrically. We found that the enzyme was produced anaerobically, as well as aerobically, and that anaerobiosis had no effect on production of this enzyme when other conditions, such as pH, were held constant.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALEXANDER M., HEPPEL L. A., HURWITZ J. The purification and properties of micrococcal nuclease. J Biol Chem. 1961 Nov;236:3014–3019. [PubMed] [Google Scholar]
- BURNS J., HOLTMAN D. F. Biochemical properties of virulent and avirulent staphylococci. Ann N Y Acad Sci. 1960 Nov 21;88:1115–1124. doi: 10.1111/j.1749-6632.1960.tb20101.x. [DOI] [PubMed] [Google Scholar]
- REDDI K. K. Mode of action of micrococcal phosphodiesterase. Nature. 1960 Jul 2;187:74–75. doi: 10.1038/187074a0. [DOI] [PubMed] [Google Scholar]
- WECKMAN B. G., CATLIN B. W. Deoxyribonuclease activity of micrococci from clinical sources. J Bacteriol. 1957 Jun;73(6):747–753. doi: 10.1128/jb.73.6.747-753.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]