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. 1968 May;95(5):1634–1641. doi: 10.1128/jb.95.5.1634-1641.1968

Origin and Sequence of Chromosome Replication in Escherichia coli B/r

Charles E Helmstetter 1
PMCID: PMC252188  PMID: 4870279

Abstract

The initial rates of induced synthesis of tryptophanase, β-galactosidase, and d-serine deaminase were measured in relation to the chromosome replication cycle of Escherichia coli B/r. Exponentially growing cultures were exposed briefly to 14C-thymidine or the appropriate inducers (or both), and the amount of label or enzyme (or both) in cells of different ages was found by measuring these quantities in their progeny. The rates of induced synthesis of the three enzymes increased abruptly at about 4, 20, and 34 min, respectively, after the start of a round of replication lasting 40 min. By matching this sequence to the ind, lac, and Dsd loci on the genetic map of E. coli K-12, it was estimated that replication began at about 8 o'clock (60 min) and proceeded clockwise. In rapidly growing cells, the sequence during the division cycle was consistent with the concept that rounds of replication overlapped.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Tomizawa J. Replication of the escherichia coli K12 chromosome. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1911–1918. doi: 10.1073/pnas.58.5.1911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Altenbern R. A. Apparent genomic mapping of Staphylococcus aureus by a new method. Biochem Biophys Res Commun. 1966 Nov 11;25(3):346–353. doi: 10.1016/0006-291x(66)90784-4. [DOI] [PubMed] [Google Scholar]
  3. BONHOEFFER F., GIERER A. ON THE GROWTH MECHANISM OF THE BACTERIAL CHROMOSOME. J Mol Biol. 1963 Nov;7:534–540. doi: 10.1016/s0022-2836(63)80100-x. [DOI] [PubMed] [Google Scholar]
  4. Boyer H. Conjugation in Escherichia coli. J Bacteriol. 1966 May;91(5):1767–1772. doi: 10.1128/jb.91.5.1767-1772.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CUMMINGS D. J. MACROMOLECULAR SYNTHESIS DURING SYNCHRONOUS GROWTH OF ESCHERICHIA COLI B/R. Biochim Biophys Acta. 1965 Feb 8;95:341–350. doi: 10.1016/0005-2787(65)90498-3. [DOI] [PubMed] [Google Scholar]
  6. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  7. Cutler R. G., Evans J. E. Relative transcription activity of different segments of the genome throughout the cell division cycle of Escherichia coli. The mapping of ribosomal and transfer RNA and the determination of the direction of replication. J Mol Biol. 1967 May 28;26(1):91–105. doi: 10.1016/0022-2836(67)90263-x. [DOI] [PubMed] [Google Scholar]
  8. DONACHIE W. D. THE REGULATION OF PYRIMIDINE BIOSYNTHESIS IN NEUROSPORA CASSA. II. HETEROKARYONS AND THE ROLE OF THE "REGULATORY MECHANISMS". Biochim Biophys Acta. 1964 Feb 10;82:293–302. doi: 10.1016/0304-4165(64)90300-9. [DOI] [PubMed] [Google Scholar]
  9. Donachie W. D., Masters M. Evidence for polarity of chromosome replication in F- strains of Escherichia coli. Genet Res. 1966 Aug;8(1):119–124. doi: 10.1017/s0016672300009976. [DOI] [PubMed] [Google Scholar]
  10. Espardellier-Joset F., Harriman P. D., Gots J., Marcovich H. Localisation de l'origine de réplication végétative chez une souche Hfr d'Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1967 Mar 13;264(11):1541–1544. [PubMed] [Google Scholar]
  11. Helmstetter C. E. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. doi: 10.1016/0022-2836(68)90424-5. [DOI] [PubMed] [Google Scholar]
  12. Helmstetter C. E., Pierucci O. Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968 May;95(5):1627–1633. doi: 10.1128/jb.95.5.1627-1633.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  14. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  15. Masters M., Kuempel P. L., Pardee A. B. Enzyme synthesis in synchronous cultures of bacteria. Biochem Biophys Res Commun. 1964 Feb 18;15(1):38–42. doi: 10.1016/0006-291x(64)90099-3. [DOI] [PubMed] [Google Scholar]
  16. Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NAGATA T. The molecular synchrony and sequential replication of DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1963 Apr;49:551–559. doi: 10.1073/pnas.49.4.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PARDEE A. B., PRESTIDGE L. S. Induced formation of serine and threonine deaminases by Escherichia coli. J Bacteriol. 1955 Dec;70(6):667–674. doi: 10.1128/jb.70.6.667-674.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. PARDEE A. B., PRESTIDGE L. S. The initial kinetics of enzyme induction. Biochim Biophys Acta. 1961 Apr 29;49:77–88. doi: 10.1016/0006-3002(61)90871-x. [DOI] [PubMed] [Google Scholar]
  20. PITTARD J., RAMAKRISHNAN T. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI. IV. EFFECT OF A CHROMOSOMAL DELETION ON CHROMOSOME TRANSFER. J Bacteriol. 1964 Aug;88:367–373. doi: 10.1128/jb.88.2.367-373.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. RYAN F. J., CETRULO S. D. DIRECTED MUTATION IN A SYNCHRONIZED BACTERIAL POPULATION. Biochem Biophys Res Commun. 1963 Aug 20;12:445–447. doi: 10.1016/0006-291x(63)90312-7. [DOI] [PubMed] [Google Scholar]
  22. Stonehill E. H., Hutchison D. J. Chromosomal mapping by means of mutational induction in synchronous populations of Streptococcus faecalis. J Bacteriol. 1966 Jul;92(1):136–143. doi: 10.1128/jb.92.1.136-143.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. YOSHIKAWA H., O'SULLIVAN A., SUEOKA N. SEQUENTIAL REPLICATION OF THE BACILLUS SUBTILIS CHROMOSOME. 3. REGULATION OF INITIATION. Proc Natl Acad Sci U S A. 1964 Oct;52:973–980. doi: 10.1073/pnas.52.4.973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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