Abstract
Enzyme extracts of Leuconostoc mesenteroides were found to contain at least four separate kinases: one active with glucose, glucosamine, and N-acetylglucosamine; one active with fructose and mannose; and two active with gluconate, one constitutive and one inducible. The molecular sizes of all the kinases, estimated from sucrose gradient centrifugation, are about the same, 37,000 to 50,000 daltons, except the constitutive gluconate kinase, which is about 100,000 daltons. Apparent Michaelis constants were calculated for all of the substrates mentioned. The kinases are separable on triethylaminoethyl cellulose.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DEMOSS R. D. A triphosphopyridine nucleotide dependent alcohol dehydrogenase from Leuconostoc mesenteroides. J Bacteriol. 1954 Aug;68(2):252–257. doi: 10.1128/jb.68.2.252-257.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson B., Westley J. Tryptophan in the active site of rhodanese. J Biol Chem. 1965 Nov;240(11):4463–4469. [PubMed] [Google Scholar]
- Kamel M. Y., Allison D. P., Anderson R. L. Stereospecific D-glucokinase of Aerobacter aerogenes. Purification and properties. J Biol Chem. 1966 Feb 10;241(3):690–694. [PubMed] [Google Scholar]
- Kenkare U. W., Colowick S. P. Reversible inactivation and dissociation of yeast hexokinase. J Biol Chem. 1965 Dec;240(12):4570–4584. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Sapico V., Anderson R. L. An adenosine 5'-triphosphate:hexose 6-phosphotransferase specific for D-mannose and D-fructose from Leuconostoc mesenteroides. Purification, properties, and evidence for a single enzyme. J Biol Chem. 1967 Nov 10;242(21):5086–5092. [PubMed] [Google Scholar]
- ZELEZNICK L. D., HANKIN H., BOLTRALIK J. J., HEYMANN H., BARKULIS S. S. PURIFICATION AND PROPERTIES OF N-ACETYL-D-GLUCOSAMINE KINASE FROM STREPTOCOCCUS PYOGENES. J Bacteriol. 1964 Nov;88:1288–1295. doi: 10.1128/jb.88.5.1288-1295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]