Abstract
Diluent composition, time consumed in experimental manipulations, and the presence of pyocine-like lethal agents affected the viability of sexual fertility factor-positive and fertility factor-negative auxotrophic stocks of Pseudomonas aeruginosa. In the same cultures, recovery of prototrophic revertants increased as the number of viable cells dispensed per plate was reduced. As a result, the number of revertants recovered was indirectly determined by the combined activities of the three conditions affecting viability. Possible modifications by these conditions affecting viability on the expression and interpretation of the fertility factor-positive sex factor-mediated system of genetic recombination are presented.
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Selected References
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- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLLOWAY B. W. Genetic recombination in Pseudomonas aeruginosa. J Gen Microbiol. 1955 Dec;13(3):572–581. doi: 10.1099/00221287-13-3-572. [DOI] [PubMed] [Google Scholar]
- JACOB F. Biosynthèse induite et mode d'action d'une pyocine, antibiotique de Pseudomonas pyocyanea. Ann Inst Pasteur (Paris) 1954 Feb;86(2):149–160. [PubMed] [Google Scholar]
- LOUTIT J. S., PEARCE L. E. MATING IN PSEUDOMONAS AERUGINOSA. Nature. 1965 Feb 20;205:822–822. doi: 10.1038/205822a0. [DOI] [PubMed] [Google Scholar]
- SHIVELY J. M., HARTSELL S. E. BACTERIOLYSIS OF THE PSEUDOMONADS. II. CHEMICAL TREATMENTS AFFECTING THE LYTIC RESPONSE. Can J Microbiol. 1964 Dec;10:911–915. doi: 10.1139/m64-118. [DOI] [PubMed] [Google Scholar]