Abstract
A methanogenic bacterium, present in bovine rumen contents at concentrations of approximately 2 × 108 cells per ml, has been isolated in pure culture. The organism is a strictly anaerobic, weakly motile, nonsporeforming, gram-negative rod (0.7 μ × 1.5 to 2.0 μ) with rounded ends. There is a single polar flagellum. The organism grows at temperatures between 30 and 45 C, with an optimum at 40 C, and at pH values between 5.9 and 7.7, with optimal growth between pH 6.1 and 6.9. Of the 17 substrates tested, only formate and H2 plus CO2 supported growth. An unidentified, heat-stable factor(s) was required by the organism. The factor, which was not one of the common ones, was present in rumen fluid, mixed rumen bacteria, and yeast extract. On the basis of colony morphology, Gram reaction, and motility, the organism is classified as a new species of methanogenic bacterium, and the name Methanobacterium mobilis sp. n. is proposed.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BEIJER W. H. Methane fermentation in the rumen of cattle. Nature. 1952 Oct 4;170(4327):576–577. doi: 10.1038/170576a0. [DOI] [PubMed] [Google Scholar]
- Burke V. Notes on the Gram Stain with Description of a New Method. J Bacteriol. 1922 Mar;7(2):159–182. doi: 10.1128/jb.7.2.159-182.1922. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUNGATE R. E. Symposium: selected topics in microbial ecology. I. Microbial ecology of the rumen. Bacteriol Rev. 1960 Dec;24(4):353–364. doi: 10.1128/br.24.4.353-364.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hungate R. E. Hydrogen as an intermediate in the rumen fermentation. Arch Mikrobiol. 1967;59(1):158–164. doi: 10.1007/BF00406327. [DOI] [PubMed] [Google Scholar]
- Hungate R. E., Smith W., Clarke R. T. Suitability of butyl rubber stoppers for closing anaerobic roll culture tubes. J Bacteriol. 1966 Feb;91(2):908–909. doi: 10.1128/jb.91.2.908-909.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH P. H., HUNGATE R. E. Isolation and characterization of Methanobacterium ruminantium n. sp. J Bacteriol. 1958 Jun;75(6):713–718. doi: 10.1128/jb.75.6.713-718.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]