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. Author manuscript; available in PMC: 2008 Aug 26.
Published in final edited form as: Biochemistry. 2006 Nov 14;45(45):13537–13542. doi: 10.1021/bi060938q

Figure 2.

Figure 2

Purification of the RecA-fused dimer protein. SDS-polyacrylamide gel (11%) showing molecular mass markers (lane M), crude extract supernatant before and after induction with IPTG (lanes 1 and 2, respectively), pooled fractions from DE52 column gradient (lane 3) and elution from ssDNA cellulose (lane 4). The final step produced RecA-FD protein at >95% purity with less than 5% contamination of monomeric RecA resulting from intracellular proteolysis (see EXPERIMENTAL PROCEDURES).