Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Jul;96(1):51–54. doi: 10.1128/jb.96.1.51-54.1968

Metabolism of d-Fructose in Aerobacter aerogenes: Analysis of Mutants Lacking d-Fructose 6-Phosphate Kinase and d-Fructose 1,6-Diphosphatase1

Virginia Sapico a, T E Hanson a,2, R W Walter a,2, R L Anderson a
PMCID: PMC252251  PMID: 4298731

Abstract

The relative significance of the pathways for the conversion of d-fructose to d-fructose 1,6-diphosphate via d-fructose 1-phosphate or d-fructose 6-phosphate in Aerobacter aerogenes PRL-R3 was assessed by observing growth patterns of mutants lacking either d-fructose 6-phosphate kinase or d-fructose 1,6-diphosphatase. The mutant lacking d-fructose 6-phosphate kinase grew well on d-fructose or glycerol but not on d-glucose, whereas the mutant lacking d-fructose 1,6-diphosphatase grew on d-glucose but not on d-fructose or glycerol. The data indicate that the pathway of d-fructose metabolism is primarily through d-fructose 1-phosphate rather than d-fructose 6-phosphate.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fraenkel D. G., Horecker B. L. Fructose-1, 6-diphosphatase and acid hexose phosphatase of Escherichia coli. J Bacteriol. 1965 Oct;90(4):837–842. doi: 10.1128/jb.90.4.837-842.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hanson T. E., Anderson R. L. D-fructose 1-phosphate kinase, a new enzyme instrumental in the metabolism of D-fructose. J Biol Chem. 1966 Apr 10;241(7):1644–1645. [PubMed] [Google Scholar]
  3. Kamel M. Y., Allison D. P., Anderson R. L. Stereospecific D-glucokinase of Aerobacter aerogenes. Purification and properties. J Biol Chem. 1966 Feb 10;241(3):690–694. [PubMed] [Google Scholar]
  4. LOVELESS A., HOWARTH S. Mutation of bacteria at high levels of survival by ethyl methane sulphonate. Nature. 1959 Dec 5;184:1780–1782. doi: 10.1038/1841780a0. [DOI] [PubMed] [Google Scholar]
  5. MAGASANIK B. Catabolite repression. Cold Spring Harb Symp Quant Biol. 1961;26:249–256. doi: 10.1101/sqb.1961.026.01.031. [DOI] [PubMed] [Google Scholar]
  6. NEIDHARDT F. C. Mutant of Aerobacter aerogenes lacking glucose repression. J Bacteriol. 1960 Oct;80:536–543. doi: 10.1128/jb.80.4.536-543.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Reeves R. E., Warren L. G., Hsu D. S. 1-Phosphofructokinase from an anaerobe. J Biol Chem. 1966 Mar 25;241(6):1257–1261. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES