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. 1968 Jul;96(1):105–110. doi: 10.1128/jb.96.1.105-110.1968

Partial Purification and Properties of a Trehalase from Streptomyces hygroscopicus

Ann E Hey a, A D Elbein a,1
PMCID: PMC252259  PMID: 5663563

Abstract

The enzyme α,α′-glucoside 1-glucohydrolase, which catalyzes the hydrolysis of trehalose, was isolated from Streptomyces hygroscopicus and was purified approximately 80-fold. The enzyme was completely specific for trehalose as substrate. None of the other naturally occurring glucose disaccharides exhibited any significant activity. The pH optimum for enzymatic activity was found to be 6.5 and the Km was estimated to be approximately 1.8 × 10−2m. The product of the reaction was identified as d-glucose by chemical, chromatographic, and enzymatic methods. The presence of this enzyme was demonstrated in several species of Streptomyces and related organisms.

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Selected References

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  1. Avigad G., Ziv O., Neufeld E. Intracellular trehalase of a hybrid yeast. Biochem J. 1965 Dec;97(3):715–722. doi: 10.1042/bj0970715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BIRCH G. G. TREHALOSES. Adv Carbohydr Chem. 1963;18:201–225. doi: 10.1016/s0096-5332(08)60243-x. [DOI] [PubMed] [Google Scholar]
  3. Ceccarini C. The biochemical relationship between trehalase and trehalose during growth and differentiation in the cellular slime mold, Dictyostelium discoideum. Biochim Biophys Acta. 1967 Oct 9;148(1):114–124. doi: 10.1016/0304-4165(67)90285-1. [DOI] [PubMed] [Google Scholar]
  4. Elbein A. D. Carbohydrate metabolism in Streptomyces hygroscopicus. I. Enzymatic synthesis of trehalose phosphate from guanosine diphosphate D-glucose-14C. J Biol Chem. 1967 Feb 10;242(3):403–406. [PubMed] [Google Scholar]
  5. Elbein A. D. Carbohydrate metabolism in streptomycetes. II. Isolation and enzymatic synthesis of trehalose. J Bacteriol. 1967 Nov;94(5):1520–1524. doi: 10.1128/jb.94.5.1520-1524.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FRIEDMAN S. The purification and properties of trehalase isolated from Phormia regina Meig. Arch Biochem Biophys. 1960 Apr;87:252–258. doi: 10.1016/0003-9861(60)90169-7. [DOI] [PubMed] [Google Scholar]
  7. HILL E. P., SUSSMAN A. S. DEVELOPMENT OF TREHALASE AND INVERTASE ACTIVITY IN NEUROSPORA. J Bacteriol. 1964 Dec;88:1556–1566. doi: 10.1128/jb.88.6.1556-1566.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HILL E. P., SUSSMAN A. S. PURIFICATION AND PROPERTIES OF TREHALASE (S) FROM NEUROSPORA. Arch Biochem Biophys. 1963 Sep;102:389–396. doi: 10.1016/0003-9861(63)90246-7. [DOI] [PubMed] [Google Scholar]
  9. KALF G. F., RIEDER S. V. The purification and properties of trehalase. J Biol Chem. 1958 Feb;230(2):691–698. [PubMed] [Google Scholar]
  10. MIYAZAWA Y., THOMAS C. A., Jr NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. J Mol Biol. 1965 Feb;11:223–237. doi: 10.1016/s0022-2836(65)80053-5. [DOI] [PubMed] [Google Scholar]
  11. PANEK A., SOUZA N. O. PURIFICATION AND PROPERTIES OF BAKERS' YEAST TREHALASE. J Biol Chem. 1964 Jun;239:1671–1673. [PubMed] [Google Scholar]
  12. Roth R., Sussman M. Trehalose synthesis in the cellular slime mold Dictyostelium discoideum. Biochim Biophys Acta. 1966 Aug 10;122(2):225–231. doi: 10.1016/0926-6593(66)90064-6. [DOI] [PubMed] [Google Scholar]
  13. SUSSMAN A. S. The role of trehalose in the activation of dormant ascospores of neurospora. Q Rev Biol. 1961 Jun;36:109–116. doi: 10.1086/403332. [DOI] [PubMed] [Google Scholar]
  14. TORII M., KABAT E. A., BEYCHOK S. ALPHA-1,2 LINKAGES IN DEXTRAN NRRL B 1424. Arch Biochem Biophys. 1963 Nov;103:283–285. doi: 10.1016/0003-9861(63)90407-7. [DOI] [PubMed] [Google Scholar]
  15. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  16. WALKER J. B., HNILICA V. S. DEVELOPMENTAL CHANGES IN ARGININE: X AMIDINOTRANSFERASE ACTIVITY IN STREPTOMYCIN-PRODUCING STRAINS OF STREPTOMYCES. Biochim Biophys Acta. 1964 Sep 18;89:473–482. doi: 10.1016/0926-6569(64)90073-2. [DOI] [PubMed] [Google Scholar]

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