Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Jul;96(1):117–125. doi: 10.1128/jb.96.1.117-125.1968

Molar Growth Yields of Certain Lactic Acid Bacteria as Influenced by Autolysis

Hassan H Moustafa 1, E B Collins 1
PMCID: PMC252261  PMID: 4969603

Abstract

Molar growth yields determined from batch cultures of Streptococcus diacetilactis and S. faecalis were appreciably greater at the peaks of maximal growth than after continued incubation and considerable autolysis. The higher molar growth yields were about equal to those determined in a continuous culture. Autolysis during logarithmic growth was minimal. The average Y value for adenosine triphosphate (ATP), determined by using limiting concentrations of glucose, galactose, lactose, and maltose for growing S. diacetilactis and limiting concentrations of glucose for growing S. lactis, S. cremoris, and S. faecalis, was 17.0. This is close to the Y (arginine) value of 17.8 determined with S. faecalis, but 62% greater than the generally accepted value of 10.5. Data are presented indicating that the often-used Y (ATP) value of 10.5 is erroneously low.

Full text

PDF
117

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAUCHOP T., ELSDEN S. R. The growth of micro-organisms in relation to their energy supply. J Gen Microbiol. 1960 Dec;23:457–469. doi: 10.1099/00221287-23-3-457. [DOI] [PubMed] [Google Scholar]
  2. Beck R. W., Shugart L. R. Molar growth yields in Streptococcus faecalis var. liquefaciens. J Bacteriol. 1966 Sep;92(3):802–803. doi: 10.1128/jb.92.3.802-803.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHEN S. L. ENERGY REQUIREMENT FOR MICROBIAL GROWTH. Nature. 1964 Jun 13;202:1135–1136. doi: 10.1038/2021135b0. [DOI] [PubMed] [Google Scholar]
  4. CITTI J. E., SANDINE W. E., ELLIKER P. R. BETA-GALACTOSIDASE OF STREPTOCOCCUS LACTIS. J Bacteriol. 1965 Apr;89:937–942. doi: 10.1128/jb.89.4.937-942.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deibel R. H. Utilization of arginine as an energy source for the growth of Streptococcus faecalis. J Bacteriol. 1964 May;87(5):988–992. doi: 10.1128/jb.87.5.988-992.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ELSDEN S. R., PEEL J. L. Metabolism of carbohydrates and related compounds. Annu Rev Microbiol. 1958;12:145–202. doi: 10.1146/annurev.mi.12.100158.001045. [DOI] [PubMed] [Google Scholar]
  7. FORREST W. W., WALKER D. J. SYNTHESIS OF RESERVE MATERIALS FOR ENDOGENOUS METABOLISM IN STREPTOCOCCUS FAECALIS. J Bacteriol. 1965 Jun;89:1448–1452. doi: 10.1128/jb.89.6.1448-1452.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HADJIPETROU L. P., STOUTHAMER A. H. AUTOLYSIS OF BACILLUS SUBTILIS BY GLUCOSE DEPLETION. Antonie Van Leeuwenhoek. 1963;29:256–260. doi: 10.1007/BF02046066. [DOI] [PubMed] [Google Scholar]
  9. HARVEY R. J., COLLINS E. B. ROLES OF CITRATE AND ACETOIN IN THE METABOLISM OF STREPTOCOCCUS DIACETILACTIS. J Bacteriol. 1963 Dec;86:1301–1307. doi: 10.1128/jb.86.6.1301-1307.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HOBSON P. N. CONTINUOUS CULTURE OF SOME ANEROBIC AND FACULTATIVELY ANAEROBIC RUMEN BACTERIA. J Gen Microbiol. 1965 Feb;38:167–180. doi: 10.1099/00221287-38-2-167. [DOI] [PubMed] [Google Scholar]
  11. HUNGATE R. E. POLYSACCHARIDE STORAGE AND GROWTH EFFICIENCY IN RUMINOCOCCUS ALBUS. J Bacteriol. 1963 Oct;86:848–854. doi: 10.1128/jb.86.4.848-854.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hobson P. N., Summers R. The continuous culture of anaerobic bacteria. J Gen Microbiol. 1967 Apr;47(1):53–65. doi: 10.1099/00221287-47-1-53. [DOI] [PubMed] [Google Scholar]
  13. KNIVETT V. A. Phosphorylation coupled with anaerobic breakdown of citrulline. Biochem J. 1954 Apr;56(4):602–606. [PMC free article] [PubMed] [Google Scholar]
  14. LARSEN D. H., DIMMICK R. L. ATTACHMENT AND GROWTH OF BACTERIA ON SURFACES OF CONTINUOUS-CULTURE VESSELS. J Bacteriol. 1964 Nov;88:1380–1387. doi: 10.1128/jb.88.5.1380-1387.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LEDERBERG J. The beta-d-galactosidase of Escherichia coli, strain K-12. J Bacteriol. 1950 Oct;60(4):381–392. doi: 10.1128/jb.60.4.381-392.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moustafa H. H., Collins E. B. Role of galactose or glucose-1-phosphate in preventing the lysis of Streptococcus diacetilactis. J Bacteriol. 1968 Feb;95(2):592–602. doi: 10.1128/jb.95.2.592-602.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. OXENBURGH M. S., SNOSWELL A. M. USE OF MOLAR GROWTH YIELDS FOR THE EVALUATION OF ENERGY-PRODUCING PATHWAYS IN LACTOBACILLUS PLANTARUM. J Bacteriol. 1965 Mar;89:913–914. doi: 10.1128/jb.89.3.913-914.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pirt S. J. The maintenance energy of bacteria in growing cultures. Proc R Soc Lond B Biol Sci. 1965 Oct 12;163(991):224–231. doi: 10.1098/rspb.1965.0069. [DOI] [PubMed] [Google Scholar]
  19. ROSENBERGER R. F., ELSDEN S. R. The yields of Streptococcus faecalis grown in continuous culture. J Gen Microbiol. 1960 Jun;22:726–739. doi: 10.1099/00221287-22-3-726. [DOI] [PubMed] [Google Scholar]
  20. ROSENBERG H., ENNOR A. H., MORRISON J. F. The estimation of arginine. Biochem J. 1956 May;63(1):153–159. doi: 10.1042/bj0630153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SENEZ J. C. Some considerations on the energetics of bacterial growth. Bacteriol Rev. 1962 Jun;26:95–107. [PMC free article] [PubMed] [Google Scholar]
  22. SLADE H. D., DOUGHTY C. C., SLAMP W. C. The synthesis of high-energy phosphate in the citrulline ureidase reaction by soluble enzymes of Pseudomonas. Arch Biochem Biophys. 1954 Feb;48(2):338–346. doi: 10.1016/0003-9861(54)90349-5. [DOI] [PubMed] [Google Scholar]
  23. STOUTHAMER A. H. Energy production in Gluconobacter liquefaciens. Biochim Biophys Acta. 1962 Jan 1;56:19–32. doi: 10.1016/0006-3002(62)90523-1. [DOI] [PubMed] [Google Scholar]
  24. Shockman G. D., Conover M. J., Kolb J. J., Phillips P. M., Riley L. S., Toennies G. LYSIS OF STREPTOCOCCUS FAECALIS. J Bacteriol. 1961 Jan;81(1):36–43. doi: 10.1128/jb.81.1.36-43.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES